Abstract
Plant endo-β-1,4-glucanases belonging to the Glycoside Hydrolase Family 9 have functional roles in cell wall biosynthesis and remodeling via endohydrolysis of (1→4)-β-d-glucosidic linkages. Modification of cell wall chemistry via RNA interference (RNAi)-mediated downregulation of Populus deltoides KORRIGAN (PdKOR), an endo-β-1,4-glucanase familygene was shown to have functional consequences on the composition of secondary metabolome and the ability of modified roots to interact with beneficial microbes. The molecular remodeling that underlies the observed differences at metabolic, physiological, and morphological levels in roots is not well understood. Here a liquid chromatography (LC)-tandem mass spectrometry (MS/MS)-based proteome profiling approach is used to survey the molecular remodeling in root tissues of PdKOR and control plants. A total of 14316 peptides are identified and these mapped to 7139 P. deltoides proteins. Based on 90% sequence identity, the measured protein accessions represent 1187 functional protein groups. Analysis of Gene Ontology (GO) categories and specific individual proteins show differential expression of proteins relevant to plant-microbe interactions, cell wall chemistry, and metabolism. The new proteome dataset serves as a useful resource for deriving new hypotheses and empirical testing pertaining to functional roles of proteins and pathways in differential priming of plant roots to interactions with microbes.
| Original language | English |
|---|---|
| Article number | 2000067 |
| Journal | Proteomics |
| Volume | 20 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 2020 |
Funding
This manuscript has been authored by UT‐Battelle, LLC under Contract No. DE‐AC05‐00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non‐exclusive, paid‐up, irrevocable, world‐wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan( http://energy.gov/downloads/doe-public-access-plan ). This research was sponsored by the Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Plant Microbe Interfaces Scientific Focus Area at Oak Ridge National Laboratory ( http://pmi.ornl.gov ). Oak Ridge National Laboratory is managed by UT‐Battelle, LLC, for the U.S. Department of Energy under contract DE‐AC05‐00OR22725.
| Funders | Funder number |
|---|---|
| U.S. Department of Energy | |
| Office of Science | |
| Biological and Environmental Research | |
| Oak Ridge National Laboratory | DE‐AC05‐00OR22725 |
Keywords
- Populus
- cell wall chemistry
- host
- metabolome
- plant-microbe interaction
- proteome
- root
- Plant Proteins/metabolism
- Plant Roots/metabolism
- Proteome/metabolism
- Tandem Mass Spectrometry
- RNA Interference
- Proteomics
- Chromatography, Liquid