Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing

J. Steven Hicks, B. W. Harker, K. L. Beattie, M. J. Doktycz

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton® MICROLAB® 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.

Original languageEnglish
Pages (from-to)878-885
Number of pages8
JournalBioTechniques
Volume30
Issue number4
DOIs
StatePublished - 2001

Funding

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