Model membrane systems used to study plasma membrane lipid asymmetry

Haden L. Scott, Kristen B. Kennison, Thais A. Enoki, Milka Doktorova, Jacob J. Kinnun, Frederick A. Heberle, John Katsaras

Research output: Contribution to journalReview articlepeer-review

26 Scopus citations

Abstract

It is well known that the lipid distribution in the bilayer leaflets of mammalian plasma membranes (PMs) is not symmetric. Despite this, model membrane studies have largely relied on chemically symmetric model membranes for the study of lipid–lipid and lipid–protein interactions. This is primarily due to the difficulty in preparing stable, asymmetric model membranes that are amenable to biophysical studies. However, in the last 20 years, efforts have been made in producing more biologically faithful model membranes. Here, we review several recently developed experimental and computational techniques for the robust generation of asymmetric model membranes and highlight a new and particularly promising technique to study membrane asymmetry.

Original languageEnglish
Article number1356
JournalSymmetry
Volume13
Issue number8
DOIs
StatePublished - Aug 2021

Funding

Funding: H.L.S., J.J.K. and J.K. are supported through the Scientific User Facilities Division of the Department of Energy (DOE) Office of Science, sponsored by the Basic Energy Science (BES) Program, DOE Office of Science, under Contract No. DEAC05-00OR22725. F.A.H., K.K. and T.A.E. are supported by NSF grant MCB-1817929 (to F.A.H.) and NIH/National Institute of General Medical Sciences grant R01GM138887 (to F.A.H.). M.D. is supported by the National Institute of General Medical Sciences of the NIH under Award Number F32GM134704.

Keywords

  • Cyclodextrin
  • Membrane asymmetry
  • Molecular dynamics simulations

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