Micropropagation of Populus trichocarpa 'Nisqually-1': The genotype deriving the Populus reference genome

Byung guk Kang; Lori Osburn; Dean Kopsell; Gerald A. Tuskan; Zong Ming Cheng

doi:10.1007/s11240-009-9596-9

Micropropagation of Populus trichocarpa 'Nisqually-1': The genotype deriving the Populus reference genome

Byung guk Kang, Lori Osburn, Dean Kopsell, Gerald A. Tuskan, Zong Ming Cheng

CBI Support Team

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31 Scopus citations

Abstract

Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populustrichocarpa (Torr. & Gray) genotype 'Nisqually-1'. However, 'Nisqually-1' has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1' was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator-free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0. 3% Gelrite^® and 5-10 g L^-1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

Original language	English
Pages (from-to)	251-257
Number of pages	7
Journal	Plant Cell, Tissue and Organ Culture
Volume	99
Issue number	3
DOIs	https://doi.org/10.1007/s11240-009-9596-9
State	Published - Nov 2009

Funding

Acknowledgments This project was supported in part by DOE-Bioenergy Center (BESC) grant, by the US Department of Energy/ Oak Ridge National Laboratory (subcontract to Z.-M.C.), and by the Tennessee Agricultural Experiment Station. The BESC is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.

Funders	Funder number
BESC
DOE Office of Science
DOE-Bioenergy Center
Office of Biological and Environmental Research
US Department of Energy
US Department of Energy Bioenergy Research Center
Oak Ridge National Laboratory
Tennessee Agricultural Experiment Station

Keywords

Activated charcoal
Cytokinin
Gelling agent
Gelrite
Poplar
Tissue culture

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10.1007/s11240-009-9596-9

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abstract = "Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populustrichocarpa (Torr. & Gray) genotype 'Nisqually-1'. However, 'Nisqually-1' has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1' was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator-free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0. 3% Gelrite{\textregistered} and 5-10 g L-1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.",

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author = "Kang, {Byung guk} and Lori Osburn and Dean Kopsell and Tuskan, {Gerald A.} and Cheng, {Zong Ming}",

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AU - Kopsell, Dean

AU - Tuskan, Gerald A.

AU - Cheng, Zong Ming

PY - 2009/11

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AB - Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populustrichocarpa (Torr. & Gray) genotype 'Nisqually-1'. However, 'Nisqually-1' has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1' was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator-free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0. 3% Gelrite® and 5-10 g L-1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

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Micropropagation of Populus trichocarpa 'Nisqually-1': The genotype deriving the Populus reference genome

Abstract

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