Microfluidics: The future of microdissection TESE?

Raheel Samuel; Odgerel Badamjav; Kristin E. Murphy; Darshan P. Patel; Jiyoung Son; Bruce K. Gale; Douglas T. Carrell; James M. Hotaling

doi:10.3109/19396368.2016.1159748

Microfluidics: The future of microdissection TESE?

Raheel Samuel
, Odgerel Badamjav
, Kristin E. Murphy
, Darshan P. Patel
, Jiyoung Son
, Bruce K. Gale
, Douglas T. Carrell
, James M. Hotaling

Research output: Contribution to journal › Review article › peer-review

33 Scopus citations

Abstract

ABSTRACT: Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.

Original language	English
Pages (from-to)	161-170
Number of pages	10
Journal	Systems Biology in Reproductive Medicine
Volume	62
Issue number	3
DOIs	https://doi.org/10.3109/19396368.2016.1159748
State	Published - May 3 2016
Externally published	Yes

Keywords

IVF
microTESE
microfluidics
sperm separation

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10.3109/19396368.2016.1159748

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title = "Microfluidics: The future of microdissection TESE?",

abstract = "ABSTRACT: Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10\% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.",

keywords = "IVF, microTESE, microfluidics, sperm separation",

author = "Raheel Samuel and Odgerel Badamjav and Murphy, \{Kristin E.\} and Patel, \{Darshan P.\} and Jiyoung Son and Gale, \{Bruce K.\} and Carrell, \{Douglas T.\} and Hotaling, \{James M.\}",

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doi = "10.3109/19396368.2016.1159748",

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T2 - The future of microdissection TESE?

AU - Samuel, Raheel

AU - Badamjav, Odgerel

AU - Murphy, Kristin E.

AU - Patel, Darshan P.

AU - Son, Jiyoung

AU - Gale, Bruce K.

AU - Carrell, Douglas T.

AU - Hotaling, James M.

PY - 2016/5/3

Y1 - 2016/5/3

N2 - ABSTRACT: Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.

AB - ABSTRACT: Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.

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KW - microfluidics

KW - sperm separation

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DO - 10.3109/19396368.2016.1159748

M3 - Review article

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EP - 170

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

IS - 3

ER -

Microfluidics: The future of microdissection TESE?

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Keywords

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