Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens

Raheel Samuel; Jiyoung Son; Timothy G. Jenkins; Alexander Jafek; Haidong Feng; Bruce K. Gale; Douglas T. Carrell; James M. Hotaling

doi:10.1016/j.urology.2019.12.053

Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens

Raheel Samuel, Jiyoung Son, Timothy G. Jenkins, Alexander Jafek, Haidong Feng, Bruce K. Gale, Douglas T. Carrell, James M. Hotaling

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Objectives: To demonstrate a novel prototype microfluidic system for rapid isolation of sperm from real and simulated microdissection testicular sperm extraction samples. Methods: The novel microfluidic system was tested using minced testicular biopsies from patients with nonobstructive azoospermia. The samples were split into 2 portions, conventional processing vs microfluidic. The embryologists were blinded to the processing protocol and searched the specimens for sperm after processing. We recorded the number of sperm found and the time to sperm identification and compared the sperm retrieval rates. Results: When compared to conventional methods, samples processed through the microfluidic system were cleaner (decreased somatic cells/debris), with the average number of sperm identified per minute improving from 1.52 sperm per minute for the control and 13.5 sperm per minute with the device yielding an 8.88 fold improvement in the sperm found per minute for the device as compared to the control. Preliminary viability and morphology tests show a minimal impact on sperm processed through the microfluidic system. Conclusion: The presented microfluidic system can facilitate rapid and efficient isolation of sperm from microdissection testicular sperm extraction samples. A prospective clinical trial to verify these results is needed to confirm this preliminary data.

Original language	English
Pages (from-to)	70-76
Number of pages	7
Journal	Urology
Volume	140
DOIs	https://doi.org/10.1016/j.urology.2019.12.053
State	Published - Jun 2020
Externally published	Yes

Funding

Funding Support: This material is based upon work supported by the National Science Foundation under grant no. 1549659 .

Funders	Funder number
National Science Foundation	1549659

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10.1016/j.urology.2019.12.053

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title = "Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens",

abstract = "Objectives: To demonstrate a novel prototype microfluidic system for rapid isolation of sperm from real and simulated microdissection testicular sperm extraction samples. Methods: The novel microfluidic system was tested using minced testicular biopsies from patients with nonobstructive azoospermia. The samples were split into 2 portions, conventional processing vs microfluidic. The embryologists were blinded to the processing protocol and searched the specimens for sperm after processing. We recorded the number of sperm found and the time to sperm identification and compared the sperm retrieval rates. Results: When compared to conventional methods, samples processed through the microfluidic system were cleaner (decreased somatic cells/debris), with the average number of sperm identified per minute improving from 1.52 sperm per minute for the control and 13.5 sperm per minute with the device yielding an 8.88 fold improvement in the sperm found per minute for the device as compared to the control. Preliminary viability and morphology tests show a minimal impact on sperm processed through the microfluidic system. Conclusion: The presented microfluidic system can facilitate rapid and efficient isolation of sperm from microdissection testicular sperm extraction samples. A prospective clinical trial to verify these results is needed to confirm this preliminary data.",

author = "Raheel Samuel and Jiyoung Son and Jenkins, {Timothy G.} and Alexander Jafek and Haidong Feng and Gale, {Bruce K.} and Carrell, {Douglas T.} and Hotaling, {James M.}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = jun,

doi = "10.1016/j.urology.2019.12.053",

language = "English",

volume = "140",

pages = "70--76",

journal = "Urology",

issn = "0090-4295",

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T1 - Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens

AU - Samuel, Raheel

AU - Son, Jiyoung

AU - Jenkins, Timothy G.

AU - Jafek, Alexander

AU - Feng, Haidong

AU - Gale, Bruce K.

AU - Carrell, Douglas T.

AU - Hotaling, James M.

PY - 2020/6

Y1 - 2020/6

N2 - Objectives: To demonstrate a novel prototype microfluidic system for rapid isolation of sperm from real and simulated microdissection testicular sperm extraction samples. Methods: The novel microfluidic system was tested using minced testicular biopsies from patients with nonobstructive azoospermia. The samples were split into 2 portions, conventional processing vs microfluidic. The embryologists were blinded to the processing protocol and searched the specimens for sperm after processing. We recorded the number of sperm found and the time to sperm identification and compared the sperm retrieval rates. Results: When compared to conventional methods, samples processed through the microfluidic system were cleaner (decreased somatic cells/debris), with the average number of sperm identified per minute improving from 1.52 sperm per minute for the control and 13.5 sperm per minute with the device yielding an 8.88 fold improvement in the sperm found per minute for the device as compared to the control. Preliminary viability and morphology tests show a minimal impact on sperm processed through the microfluidic system. Conclusion: The presented microfluidic system can facilitate rapid and efficient isolation of sperm from microdissection testicular sperm extraction samples. A prospective clinical trial to verify these results is needed to confirm this preliminary data.

AB - Objectives: To demonstrate a novel prototype microfluidic system for rapid isolation of sperm from real and simulated microdissection testicular sperm extraction samples. Methods: The novel microfluidic system was tested using minced testicular biopsies from patients with nonobstructive azoospermia. The samples were split into 2 portions, conventional processing vs microfluidic. The embryologists were blinded to the processing protocol and searched the specimens for sperm after processing. We recorded the number of sperm found and the time to sperm identification and compared the sperm retrieval rates. Results: When compared to conventional methods, samples processed through the microfluidic system were cleaner (decreased somatic cells/debris), with the average number of sperm identified per minute improving from 1.52 sperm per minute for the control and 13.5 sperm per minute with the device yielding an 8.88 fold improvement in the sperm found per minute for the device as compared to the control. Preliminary viability and morphology tests show a minimal impact on sperm processed through the microfluidic system. Conclusion: The presented microfluidic system can facilitate rapid and efficient isolation of sperm from microdissection testicular sperm extraction samples. A prospective clinical trial to verify these results is needed to confirm this preliminary data.

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SN - 0090-4295

VL - 140

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JO - Urology

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ER -

Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens

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