Microdissection and measurement of polytene chromosomes using the atomic force microscope

C. Mosher, D. Jondle, L. Ambrosio, J. Vesenka, E. Henderson, T. M. Seed, H. Hansma, E. De Harven, N. F. Van Hulst, R. J. Warmack

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A method to isolate specific regions of the Drosophila polytene chromosome using an atomic force microscope (AFM) was explored. The AFM was used for the microdissection of the locus of interest with much greater precision than standard microdissection techniques. The amplification of DNA isolated in this fashion by the polymerase chain reaction (PCR) is discussed. A study of the effect of hydration level on gross chromosome structure was carried out. It was shown that chromosome swelling is dependent upon humidity or the buffered medium. The significance of this swelling with respect to studies of chromosome structure under physiological conditions is considered.

Original languageEnglish
Pages (from-to)491-497
Number of pages7
JournalScanning Microscopy
Volume8
Issue number3
StatePublished - 1994
Externally publishedYes

Keywords

  • Atomic Force Microscope
  • humidity
  • microdissection
  • polytene chromosome

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