Microbial oxidation and bioluminescence response for toluene and trichloroethylene

Kevin G. Robinson, Jan G. Pieters, John Sanseverino, Chris D. Cox, Charles L. Wright, Catherine L. Cheng, Gary S. Sayler

Research output: Contribution to journalConference articlepeer-review

7 Scopus citations

Abstract

Two Pseudomonas strains, P. putida F1 and P. putida TVA8, were used to evaluate biodegradation of toluene and cometabolism of trichloroethylene (TCE) in batch systems. Both organisms effectively utilized toluene although at different rates. The extent of TCE cometabolism was dependent upon the initial mass of toluene present. A relationship between toluene/TCE degradation and bioluminescence production for P. putida TVA8 was explored. A linear relationship between the total amount of toluene degraded and total bioluminescence was found. The slope of this straight line, however, was found to vary between experiments. To overcome this problem, a normalization method was proposed and successful tested.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalWater Science and Technology
Volume38
Issue number7 pt 6
DOIs
StatePublished - 1998
Externally publishedYes
EventProceedings of the 1998 19th Biennial Conference of the International Association on Water Quality. Part 1 (of 9) - Vancouver, Can
Duration: Jun 21 1998Jun 26 1998

Keywords

  • Bioluminescence
  • Bioremediation
  • Pseudomonas putida
  • Toluene
  • Trichloroethylene

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