Microbial bioluminescent sensing of bioavailable contaminants in environmental matrices

J. Sanseverino, S. Ripp, A. Layton, G. S. Sayler

Research output: Contribution to journalArticlepeer-review

Abstract

A paper summarizes recent developments in bioreporter technology and field work monitoring naphthalene, toluene, and TCE in contaminated aquifers; bioavailability, which is the ability of a compound to be freely transported across the cell membrane for intracellular metabolism; direct measurement of specific hydrophobic organic compounds (HOC) in real time using prokaryotic bioluminescent bioreporters; use of Pseudomonas fluorescens HK44 (nah-lux; naphthalene bioreporter), P. fluorescens 5RL (nah-lux; naphthalene bioreporter), P. putida TVA8 (tod-lux; toluene and TCE), Ralstonia eutropha JMP134 (tfd-lux; 2,4-dichlrophenoxyacetic acid and 2,4-dichlorophenol), and ralstonia eutropha ENV307 (bph-lux; biphenyl and monochlorinated biphenyls) in soils and aquifer samples in the laboratory and field; and portable photomultiplier-based systems and complementary metal oxide semiconductor microluminometers developed to monitor bioluminescence from soil-born microorganisms or directly from encapsulated bioreceptors.

Original languageEnglish
Pages (from-to)483-484
Number of pages2
JournalSoil and Sediment Contamination
Volume11
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

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