Method for obtaining structure and interactions from oriented lipid bilayers

Yulia Lyatskaya, Yufeng Liu, Stephanie Tristram-Nagle, John Katsaras, John F. Nagle

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Precise calculations are made of the scattering intensity (Formula presented) from an oriented stack of lipid bilayers using a realistic model of fluctuations. The quantities of interest include the bilayer bending modulus (Formula presented) the interbilayer interaction modulus B, and bilayer structure through the form factor (Formula presented) It is shown how (Formula presented) and B may be obtained from data at large (Formula presented) where fluctuations dominate. Good estimates of (Formula presented) can be made over wide ranges of (Formula presented) by using (Formula presented) in (Formula presented) regions away from the peaks and for (Formula presented) where details of the scattering domains play little role. Rough estimates of domain sizes can also be made from smaller (Formula presented) data. Results are presented for data taken on fully hydrated, oriented DOPC bilayers in the (Formula presented) phase. These results illustrate the advantages of oriented samples compared to powder samples.

Original languageEnglish
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume63
Issue number1
DOIs
StatePublished - 2001

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