Abstract
Sphaerulina musiva is an economically and ecologically important fungal pathogen that causes Septoria stem canker and leaf spot disease of Populus species. To bridge the gap between genetic markers and structural barriers previously found to be linked to Septoria canker disease resistance in poplar, we used hydrophilic interaction liquid chromatography and tandem mass spectrometry to identify and quantify metabolites involved with signaling and cell wall remodeling. Fluctuations in signaling molecules, organic acids, amino acids, sterols, phenolics, and saccharides in resistant and susceptible P. trichocarpa inoculated with S. musiva were observed. The patterns of 222 metabolites in the resistant host implicate systemic acquired resistance (SAR), cell wall apposition, and lignin deposition as modes of resistance to this hemibiotrophic pathogen. This pattern is consistent with the expected response to the biotrophic phase of S. musiva colonization during the first 24 h postinoculation. The fungal pathogen metabolized key regulatory signals of SAR, other phenolics, and precursors of lignin biosynthesis that were depleted in the susceptible host. This is the first study to characterize metabolites associated with the response to initial colonization by S. musiva between resistant and susceptible hosts.
Original language | English |
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Pages (from-to) | 2052-2066 |
Number of pages | 15 |
Journal | Phytopathology |
Volume | 111 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2021 |
Funding
Funding: This study was supported by the U.S. Department of Energy (DOE) Office of Science, Office of Biological and Environmental Research (BER), grant DE-SC0018196 to J. M. LeBoldus; the DOE Office of Science, BER, grant DE-SC0018196; the Plant–Microbe Interfaces Scientific Focus Area in the Genomic Science Program; and the DOE BioEnergy Science Center project. The BioEn-ergy Science Center is a U.S. DOE Bioenergy Research Center supported by the Office of BER in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the U.S. DOE under contract number DE-AC05-00OR22725. S. S. Galanie recognizes support from the Center for Bio-energy Innovation, a U.S. DOE Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The work conducted by the U.S. DOE Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. DOE under contract DE-AC02-05CH11231. This study was supported by the U.S. Department of Energy (DOE) Office of Science, Office of Biological and Environmental Research (BER), grant DE-SC0018196 to J. M. LeBoldus; the DOE Office of Science, BER, grant DE-SC0018196; the Plant?Microbe Interfaces Scientific Focus Area in the Genomic Science Program; and the DOE BioEnergy Science Center project. The BioEnergy Science Center is a U.S. DOE Bioenergy Research Center supported by the Office of BER in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the U.S. DOE under contract number DE-AC05-00OR22725. S. S. Galanie recognizes support from the Center for Bioenergy Innovation, a U.S. DOE Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The work conducted by the U.S. DOE Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. DOE under contract DE-AC02-05CH11231.