Metabolism differences of cembratrien-diols in different tobacco cultivars

Compositions of leaf surface exudates of different tobacco varieties and changes of cembratrien-diols were studied, sensory quality of flue-cured tobacco leaves was also evaluated in order to provide an effective method of finding new tobacco species resources with special aroma. Surface exudates compositions in 60-day-age and mature stage cutters of 7 tobacco varieties, namely 8306, 12451, Yunyan87, Y892×8306, Yuyan No. 11, NX232 and Zhongyan100 were determined with GC-MS method. Expression level of CYC-1 and CYP71D16, solanone content and sensory quality in flue-cured tobacco leaves was analyzed. Results showed that 13 flavor substances in compositions of leaf surface exudates in 60-day-age cutters of the 7 tobacco varieties were detected with cembratriene-diols being the main substance. The content of cembratriene-diols, being the main flavor composition, varied among varieties with the highest content in 8306 at 60 days after leaf sprouting, followed by Yuyan No. 11 and 12451.Expression level of CYC-1 and CYP71D16 in 8306 was significantly higher than that in 12451 and Yunyan87 while8306 and Yuyan No. 11 had higher content of solanone.