Mercury methylation by HgcA: Theory supports carbanion transfer to Hg(II)

Jing Zhou, Demian Riccardi, Ariana Beste, Jeremy C. Smith, Jerry M. Parks

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Many proteins use corrinoid cofactors to facilitate methyl transfer reactions. Recently, a corrinoid protein, HgcA, has been shown to be required for the production of the neurotoxin methylmercury by anaerobic bacteria. A strictly conserved Cys residue in HgcA was predicted to be a lower-axial ligand to Co(III), which has never been observed in a corrinoid protein. Here, we use density functional theory to study homolytic and heterolytic Co-C bond dissociation and methyl transfer to Hg(II) substrates with model methylcobalamin complexes containing a lower-axial Cys or His ligand to cobalt, the latter of which is commonly found in other corrinoid proteins. We find that Cys thiolate coordination to Co facilitates both methyl radical and methyl carbanion transfer to Hg(II) substrates, but carbanion transfer is more favorable overall in the condensed phase. Thus, our findings are consistent with HgcA representing a new class of corrinoid protein capable of transferring methyl groups to electrophilic substrates.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalInorganic Chemistry
Volume53
Issue number2
DOIs
StatePublished - Jan 21 2014

Funding

FundersFunder number
U.S. Department of EnergyDE-SC0004895

    Fingerprint

    Dive into the research topics of 'Mercury methylation by HgcA: Theory supports carbanion transfer to Hg(II)'. Together they form a unique fingerprint.

    Cite this