Maternal consumption of fish oil programs reduced adiposity in broiler chicks

Ronique C. Beckford; Sarah J. Howard; Suchita Das; Abigail T. Farmer; Shawn R. Campagna; Jiali Yu; Robert L. Hettich; Jeanna L. Wilson; Brynn H. Voy

doi:10.1038/s41598-017-13519-5

Maternal consumption of fish oil programs reduced adiposity in broiler chicks

Ronique C. Beckford, Sarah J. Howard, Suchita Das, Abigail T. Farmer, Shawn R. Campagna, Jiali Yu, Robert L. Hettich, Jeanna L. Wilson, Brynn H. Voy

Bioanalytical Mass Spectrometry

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Maternal intake of eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (22:6 n-3) has been associated with reduced adiposity in children, suggesting the possibility to program adipose development through dietary fatty acids before birth. This study determined if enriching the maternal diet in fish oil, the primary source of EPA and DHA, affected adipose development in offspring. Broiler chickens were used because they are obesity-prone, and because fatty acids provided to the embryo can be manipulated through the hen diet. Hens were fed diets supplemented (2.8% wt:wt) with corn oil (CO; n-6) or fish oil (FO; n-3) for 28 d. Chicks from both maternal diet groups were fed the same diet after hatch. Maternal FO consumption enriched chick adipose tissue in EPA and DHA and reduced adiposity by promoting more, but smaller, adipocytes. This adipocyte profile was paralleled by upregulated expression of the adipogenic regulator PPARG and its co-activator PPARGC1B, and reduced expression of LPL. Proteomics identified 95 differentially abundant proteins between FO and CO adipose tissue, including components of glucose metabolism, lipid droplet trafficking, and cytoskeletal organization. These results demonstrate that the maternal dietary fatty acid profile programs offspring adipose development.

Original language	English
Article number	13129
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-13519-5
State	Published - Dec 1 2017

Funding

This work was supported by funding to B.H.V. from the Center of Excellence in Livestock Diseases and Human Health, University of Tennessee College of Veterinary Medicine, and by AgResearch, University of Tennessee Institute of Agriculture. The authors would like to acknowledge the W.M. Keck Metabolomics Research Laboratory, Iowa State University, for the fatty acid analyses and to thank Dr. Michael O. Smith and Dr. Jay Whelan for their input into the project.

Funders	Funder number
Center of Excellence in Livestock Diseases and Human Health
AgResearch
Institute of Agriculture, University of Tennessee
College of Veterinary Medicine, University of Tennessee

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41598-017-13519-5

Cite this

@article{0844095fdeb04ab8b688df24e42f56cc,

title = "Maternal consumption of fish oil programs reduced adiposity in broiler chicks",

abstract = "Maternal intake of eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (22:6 n-3) has been associated with reduced adiposity in children, suggesting the possibility to program adipose development through dietary fatty acids before birth. This study determined if enriching the maternal diet in fish oil, the primary source of EPA and DHA, affected adipose development in offspring. Broiler chickens were used because they are obesity-prone, and because fatty acids provided to the embryo can be manipulated through the hen diet. Hens were fed diets supplemented (2.8% wt:wt) with corn oil (CO; n-6) or fish oil (FO; n-3) for 28 d. Chicks from both maternal diet groups were fed the same diet after hatch. Maternal FO consumption enriched chick adipose tissue in EPA and DHA and reduced adiposity by promoting more, but smaller, adipocytes. This adipocyte profile was paralleled by upregulated expression of the adipogenic regulator PPARG and its co-activator PPARGC1B, and reduced expression of LPL. Proteomics identified 95 differentially abundant proteins between FO and CO adipose tissue, including components of glucose metabolism, lipid droplet trafficking, and cytoskeletal organization. These results demonstrate that the maternal dietary fatty acid profile programs offspring adipose development.",

author = "Beckford, {Ronique C.} and Howard, {Sarah J.} and Suchita Das and Farmer, {Abigail T.} and Campagna, {Shawn R.} and Jiali Yu and Hettich, {Robert L.} and Wilson, {Jeanna L.} and Voy, {Brynn H.}",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-13519-5",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - Maternal consumption of fish oil programs reduced adiposity in broiler chicks

AU - Beckford, Ronique C.

AU - Howard, Sarah J.

AU - Das, Suchita

AU - Farmer, Abigail T.

AU - Campagna, Shawn R.

AU - Yu, Jiali

AU - Hettich, Robert L.

AU - Wilson, Jeanna L.

AU - Voy, Brynn H.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Maternal intake of eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (22:6 n-3) has been associated with reduced adiposity in children, suggesting the possibility to program adipose development through dietary fatty acids before birth. This study determined if enriching the maternal diet in fish oil, the primary source of EPA and DHA, affected adipose development in offspring. Broiler chickens were used because they are obesity-prone, and because fatty acids provided to the embryo can be manipulated through the hen diet. Hens were fed diets supplemented (2.8% wt:wt) with corn oil (CO; n-6) or fish oil (FO; n-3) for 28 d. Chicks from both maternal diet groups were fed the same diet after hatch. Maternal FO consumption enriched chick adipose tissue in EPA and DHA and reduced adiposity by promoting more, but smaller, adipocytes. This adipocyte profile was paralleled by upregulated expression of the adipogenic regulator PPARG and its co-activator PPARGC1B, and reduced expression of LPL. Proteomics identified 95 differentially abundant proteins between FO and CO adipose tissue, including components of glucose metabolism, lipid droplet trafficking, and cytoskeletal organization. These results demonstrate that the maternal dietary fatty acid profile programs offspring adipose development.

AB - Maternal intake of eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (22:6 n-3) has been associated with reduced adiposity in children, suggesting the possibility to program adipose development through dietary fatty acids before birth. This study determined if enriching the maternal diet in fish oil, the primary source of EPA and DHA, affected adipose development in offspring. Broiler chickens were used because they are obesity-prone, and because fatty acids provided to the embryo can be manipulated through the hen diet. Hens were fed diets supplemented (2.8% wt:wt) with corn oil (CO; n-6) or fish oil (FO; n-3) for 28 d. Chicks from both maternal diet groups were fed the same diet after hatch. Maternal FO consumption enriched chick adipose tissue in EPA and DHA and reduced adiposity by promoting more, but smaller, adipocytes. This adipocyte profile was paralleled by upregulated expression of the adipogenic regulator PPARG and its co-activator PPARGC1B, and reduced expression of LPL. Proteomics identified 95 differentially abundant proteins between FO and CO adipose tissue, including components of glucose metabolism, lipid droplet trafficking, and cytoskeletal organization. These results demonstrate that the maternal dietary fatty acid profile programs offspring adipose development.

UR - http://www.scopus.com/inward/record.url?scp=85031684619&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-13519-5

DO - 10.1038/s41598-017-13519-5

M3 - Article

C2 - 29030616

AN - SCOPUS:85031684619

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 13129

ER -

Maternal consumption of fish oil programs reduced adiposity in broiler chicks

Abstract

Funding

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this