Mass spectrometric approaches for characterizing bacterial proteomes

Nathan C. VerBerkmoes; Heather M. Connelly; Chongle Pan; Robert L. Hettich

doi:10.1586/14789450.1.4.433

Mass spectrometric approaches for characterizing bacterial proteomes

Nathan C. VerBerkmoes, Heather M. Connelly, Chongle Pan, Robert L. Hettich

Bioanalytical Mass Spectrometry

Research output: Contribution to journal › Review article › peer-review

15 Scopus citations

Abstract

The emergence of advanced liquid chromatography mass spectrometry technologies for characterizing very complex mixtures of proteins has greatly propelled the field of proteomics, the goal of which is the simultaneous examination of all the proteins expressed by an organism. This research area represents a paradigm shift in molecular biology by attempting to provide a top-down qualitative and quantitative view of all the proteins (including their modifications and interactions) that are essential for an organism's life cycle, rather than targeting a particular protein family. This level of global protein information about an organism such as a bacterium can be combined with genomic and metabolomic data to enable a systems biology approach for understanding how these organisms live and function.

Original language	English
Pages (from-to)	433-447
Number of pages	15
Journal	Expert Review of Proteomics
Volume	1
Issue number	4
DOIs	https://doi.org/10.1586/14789450.1.4.433
State	Published - 2004

Funding

This research was sponsored by the Office of Biological and Environmental Sciences, US Department of Energy. Oak Ridge National Laboratory is operated and managed for the US Department of Energy by the University of Tennessee-Battelle, LLC under contract DE-AC05-00OR22725.

Funders	Funder number
Office of Biological and Environmental Sciences
U.S. Department of Energy
University of Tennessee-Battelle	DE-AC05-00OR22725

Keywords

Bacteria
Bottom-up MS
Liquid chromatography
Mass spectrometry
Peptide
Protein
Proteome
Tandem mass spectrometry
Top-down MS

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10.1586/14789450.1.4.433

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AU - Pan, Chongle

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KW - Mass spectrometry

KW - Peptide

KW - Protein

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KW - Tandem mass spectrometry

KW - Top-down MS

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Mass spectrometric approaches for characterizing bacterial proteomes

Abstract

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