Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

Miaomiao Li; Beibei Li; Guanghao Guo; Yongxing Chen; Jingzhong Xie; Ping Lu; Qiuhong Wu; Deyun Zhang; Huaizhi Zhang; Jian Yang; Panpan Zhang; Yan Zhang; Zhiyong Liu

doi:10.1016/j.cj.2018.01.004

Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

Miaomiao Li, Beibei Li, Guanghao Guo, Yongxing Chen, Jingzhong Xie, Ping Lu, Qiuhong Wu, Deyun Zhang, Huaizhi Zhang, Jian Yang, Panpan Zhang, Yan Zhang, Zhiyong Liu

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.

Original language	English
Pages (from-to)	236-243
Number of pages	8
Journal	Crop Journal
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/j.cj.2018.01.004
State	Published - Jun 2018
Externally published	Yes

Keywords

Bulk segregant analysis
Genetic mapping
Leaf senescence
Triticum aestivum

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10.1016/j.cj.2018.01.004

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title = "Mapping a leaf senescence gene els1 by BSR-Seq in common wheat",

abstract = "Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.",

keywords = "Bulk segregant analysis, Genetic mapping, Leaf senescence, Triticum aestivum",

author = "Miaomiao Li and Beibei Li and Guanghao Guo and Yongxing Chen and Jingzhong Xie and Ping Lu and Qiuhong Wu and Deyun Zhang and Huaizhi Zhang and Jian Yang and Panpan Zhang and Yan Zhang and Zhiyong Liu",

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year = "2018",

month = jun,

doi = "10.1016/j.cj.2018.01.004",

language = "English",

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AU - Li, Miaomiao

AU - Li, Beibei

AU - Guo, Guanghao

AU - Chen, Yongxing

AU - Xie, Jingzhong

AU - Lu, Ping

AU - Wu, Qiuhong

AU - Zhang, Deyun

AU - Zhang, Huaizhi

AU - Yang, Jian

AU - Zhang, Panpan

AU - Zhang, Yan

AU - Liu, Zhiyong

PY - 2018/6

Y1 - 2018/6

N2 - Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.

AB - Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence (els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.

KW - Bulk segregant analysis

KW - Genetic mapping

KW - Leaf senescence

KW - Triticum aestivum

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DO - 10.1016/j.cj.2018.01.004

M3 - Article

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SN - 2095-5421

VL - 6

SP - 236

EP - 243

JO - Crop Journal

JF - Crop Journal

IS - 3

ER -

Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

Abstract

Keywords

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