Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering

Renáta Ünnep; Ottó Zsiros; Zsolt Hörcsik; Márton Markó; Anjana Jajoo; Joachim Kohlbrecher; Győző Garab; Gergely Nagy

doi:10.1016/j.bbabio.2017.02.010

Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering

Renáta Ünnep, Ottó Zsiros, Zsolt Hörcsik, Márton Markó, Anjana Jajoo, Joachim Kohlbrecher, Győző Garab, Gergely Nagy

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p²H (pD) and show that gradually lowering the p²H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity — similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.

Original language	English
Pages (from-to)	360-365
Number of pages	6
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1858
Issue number	5
DOIs	https://doi.org/10.1016/j.bbabio.2017.02.010
State	Published - May 1 2017
Externally published	Yes

Funding

This work is based on experiments performed on the SANS-II beam-line at the Swiss spallation neutron source SINQ, Paul Scherrer Institute, Villigen, Switzerland. This work was supported by grants from the Hungarian Scientific Research Fund (NKFIH OTKA K 112688, GINOP-2.3.2-15-2016-00001 to GG) and Scientific Exchange Program-NMS·CH (SCIEX-Project number 13.098 to RÜ). GG acknowledges partial support from the Moravian-Silesian Region (Project title: Strengthening international cooperation in science, research and education; ID: 01211/2016/RRC).

Funders	Funder number
Moravian-Silesian Region	01211/2016/RRC
NKFIH OTKA	13.098, GINOP-2.3.2-15-2016-00001, K 112688
Hungarian Scientific Research Fund

Keywords

Chloroplast thylakoid membranes
Lamellar repeat distance
Low pH and pH
Small-angle neutron scattering (SANS)

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10.1016/j.bbabio.2017.02.010

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title = "Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering",

abstract = "Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H (pD) and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity — similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.",

keywords = "Chloroplast thylakoid membranes, Lamellar repeat distance, Low pH and pH, Small-angle neutron scattering (SANS)",

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AU - Ünnep, Renáta

AU - Zsiros, Ottó

AU - Hörcsik, Zsolt

AU - Markó, Márton

AU - Jajoo, Anjana

AU - Kohlbrecher, Joachim

AU - Garab, Győző

AU - Nagy, Gergely

PY - 2017/5/1

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N2 - Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H (pD) and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity — similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.

AB - Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H (pD) and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity — similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.

KW - Chloroplast thylakoid membranes

KW - Lamellar repeat distance

KW - Low pH and pH

KW - Small-angle neutron scattering (SANS)

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Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering

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