Lipid membranes loaded with Ca2+ and Zn2+ cations

N. Kučerka; E. Dushanov; K. T. Kholmurodov; J. Katsaras; D. Uhríková

doi:10.1088/1742-6596/848/1/012008

Lipid membranes loaded with Ca²⁺ and Zn²⁺ cations

N. Kučerka
, E. Dushanov
, K. T. Kholmurodov
, J. Katsaras
, D. Uhríková

Labs & Soft Matter

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

We have studied the interactions of calcium and zinc with the biomimetic membrane made of dipalmitoyl-phosphatidylcholine (DPPC). The small angle neutron experiment on oriented multilamellar samples showed clearly differences in the effects of the two cations. For both, a bilayer thickness increases due to divalent metal ion (Me²⁺) binding, reaching the maximum at stoichiometry Me²⁺:DPPC∼1:7 mol/mol. However, while the further increase in Ca²⁺ results in a bilayer thinning down to the level of pure DPPC, the Zn²⁺ binding indicates the behaviour of a typical isotherm, reaching a level of saturation.

Original language	English
Article number	012008
Journal	Journal of Physics: Conference Series
Volume	848
Issue number	1
DOIs	https://doi.org/10.1088/1742-6596/848/1/012008
State	Published - Jun 8 2017
Event	3rd International Conference on Small Angle Neutron Scattering Dedicated to the 80th Anniversary of Yu.M. Ostanevich, YuMO 2016 - Dubna, Russian Federation Duration: Jun 6 2016 → Jun 9 2016

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title = "Lipid membranes loaded with Ca2+ and Zn2+ cations",

abstract = "We have studied the interactions of calcium and zinc with the biomimetic membrane made of dipalmitoyl-phosphatidylcholine (DPPC). The small angle neutron experiment on oriented multilamellar samples showed clearly differences in the effects of the two cations. For both, a bilayer thickness increases due to divalent metal ion (Me2+) binding, reaching the maximum at stoichiometry Me2+:DPPC∼1:7 mol/mol. However, while the further increase in Ca2+ results in a bilayer thinning down to the level of pure DPPC, the Zn2+ binding indicates the behaviour of a typical isotherm, reaching a level of saturation.",

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note = "Publisher Copyright: {\textcopyright} Published under licence by IOP Publishing Ltd.; 3rd International Conference on Small Angle Neutron Scattering Dedicated to the 80th Anniversary of Yu.M. Ostanevich, YuMO 2016 ; Conference date: 06-06-2016 Through 09-06-2016",

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T1 - Lipid membranes loaded with Ca2+ and Zn2+ cations

AU - Kučerka, N.

AU - Dushanov, E.

AU - Kholmurodov, K. T.

AU - Katsaras, J.

AU - Uhríková, D.

N1 - Publisher Copyright: © Published under licence by IOP Publishing Ltd.

PY - 2017/6/8

Y1 - 2017/6/8

N2 - We have studied the interactions of calcium and zinc with the biomimetic membrane made of dipalmitoyl-phosphatidylcholine (DPPC). The small angle neutron experiment on oriented multilamellar samples showed clearly differences in the effects of the two cations. For both, a bilayer thickness increases due to divalent metal ion (Me2+) binding, reaching the maximum at stoichiometry Me2+:DPPC∼1:7 mol/mol. However, while the further increase in Ca2+ results in a bilayer thinning down to the level of pure DPPC, the Zn2+ binding indicates the behaviour of a typical isotherm, reaching a level of saturation.

AB - We have studied the interactions of calcium and zinc with the biomimetic membrane made of dipalmitoyl-phosphatidylcholine (DPPC). The small angle neutron experiment on oriented multilamellar samples showed clearly differences in the effects of the two cations. For both, a bilayer thickness increases due to divalent metal ion (Me2+) binding, reaching the maximum at stoichiometry Me2+:DPPC∼1:7 mol/mol. However, while the further increase in Ca2+ results in a bilayer thinning down to the level of pure DPPC, the Zn2+ binding indicates the behaviour of a typical isotherm, reaching a level of saturation.

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DO - 10.1088/1742-6596/848/1/012008

M3 - Conference article

AN - SCOPUS:85022029070

SN - 1742-6588

VL - 848

JO - Journal of Physics: Conference Series

JF - Journal of Physics: Conference Series

IS - 1

M1 - 012008

T2 - 3rd International Conference on Small Angle Neutron Scattering Dedicated to the 80th Anniversary of Yu.M. Ostanevich, YuMO 2016

Y2 - 6 June 2016 through 9 June 2016

ER -

Lipid membranes loaded with Ca²⁺ and Zn²⁺ cations

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