LIF imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes

Alexander A. Puretzky; David B. Geohegan

doi:10.1016/S0169-4332(97)00639-9

LIF imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes

Alexander A. Puretzky, David B. Geohegan

Functional Hybrid Nanomaterials

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The first gated laser-induced fluorescence (LIF) imaging and absorption spectroscopy have been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (matrix assisted laser desorption ionization) matrices. These plasma diagnostic techniques, as well as ion probe measurements, were employed to investigate the desorbed products, densities, fractional ionization, and velocity distributions of the plume of ejecta which is typically encountered as the main desorption product in the mass spectrometry analysis of large biomolecules. Pulsed-ultraviolet (193 nm and 248 nm) desorption from 3-hydroxypicolinic acid (3-HPA) crystals was studied. Gas-phase absorption spectra of 3-HPA measured here revealed very different absorption cross-sections at these two wavelengths, and this was exploited to investigate the effects of plume absorption of the desorbing laser pulse. In both cases, LIF imaging revealed two plume components: a fast (maximum ∼ 0.1 cm / μs) low-intensity component which appear to be 3-HPA fragments, and a slower component of 3-HPA expanding at 0.05 cm / μs. In the case of ArF-laser irradiation, optical absorption spectroscopy indicated a breaking of the intramolecular hydrogen bond in the gas-phase matrix material.

Original language	English
Pages (from-to)	248-254
Number of pages	7
Journal	Applied Surface Science
Volume	127-129
DOIs	https://doi.org/10.1016/S0169-4332(97)00639-9
State	Published - May 1998

Funding

The authors gratefully acknowledge assistance in sample preparation and many helpful discussions with G. Hurst and M. Buchanan and the assistance of B. Sales and L. Boatner with optical and scanning electron microscopy. This research was sponsored by the Laboratory Directed Research Development Program at Oak Ridge National Laboratory, managed by Lockheed Martin Energy Research, for the US Department of Energy, under contract DE-AC05-96OR22464. A.A.P. was supported by an appointment to the ORNL Postdoctoral Research Associates Program administered jointly by ORNL and the Oak Ridge Institute for Science and Education.

Funders	Funder number
Lockheed Martin Energy Research
U.S. Department of Energy	DE-AC05-96OR22464
Oak Ridge National Laboratory
Oak Ridge Institute for Science and Education

Keywords

Laser
Laser-induced fluorescence
Plasma diagnostic technique

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10.1016/S0169-4332(97)00639-9

Cite this

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title = "LIF imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes",

abstract = "The first gated laser-induced fluorescence (LIF) imaging and absorption spectroscopy have been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (matrix assisted laser desorption ionization) matrices. These plasma diagnostic techniques, as well as ion probe measurements, were employed to investigate the desorbed products, densities, fractional ionization, and velocity distributions of the plume of ejecta which is typically encountered as the main desorption product in the mass spectrometry analysis of large biomolecules. Pulsed-ultraviolet (193 nm and 248 nm) desorption from 3-hydroxypicolinic acid (3-HPA) crystals was studied. Gas-phase absorption spectra of 3-HPA measured here revealed very different absorption cross-sections at these two wavelengths, and this was exploited to investigate the effects of plume absorption of the desorbing laser pulse. In both cases, LIF imaging revealed two plume components: a fast (maximum ∼ 0.1 cm / μs) low-intensity component which appear to be 3-HPA fragments, and a slower component of 3-HPA expanding at 0.05 cm / μs. In the case of ArF-laser irradiation, optical absorption spectroscopy indicated a breaking of the intramolecular hydrogen bond in the gas-phase matrix material.",

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author = "Puretzky, {Alexander A.} and Geohegan, {David B.}",

year = "1998",

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doi = "10.1016/S0169-4332(97)00639-9",

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AU - Puretzky, Alexander A.

AU - Geohegan, David B.

PY - 1998/5

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N2 - The first gated laser-induced fluorescence (LIF) imaging and absorption spectroscopy have been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (matrix assisted laser desorption ionization) matrices. These plasma diagnostic techniques, as well as ion probe measurements, were employed to investigate the desorbed products, densities, fractional ionization, and velocity distributions of the plume of ejecta which is typically encountered as the main desorption product in the mass spectrometry analysis of large biomolecules. Pulsed-ultraviolet (193 nm and 248 nm) desorption from 3-hydroxypicolinic acid (3-HPA) crystals was studied. Gas-phase absorption spectra of 3-HPA measured here revealed very different absorption cross-sections at these two wavelengths, and this was exploited to investigate the effects of plume absorption of the desorbing laser pulse. In both cases, LIF imaging revealed two plume components: a fast (maximum ∼ 0.1 cm / μs) low-intensity component which appear to be 3-HPA fragments, and a slower component of 3-HPA expanding at 0.05 cm / μs. In the case of ArF-laser irradiation, optical absorption spectroscopy indicated a breaking of the intramolecular hydrogen bond in the gas-phase matrix material.

AB - The first gated laser-induced fluorescence (LIF) imaging and absorption spectroscopy have been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (matrix assisted laser desorption ionization) matrices. These plasma diagnostic techniques, as well as ion probe measurements, were employed to investigate the desorbed products, densities, fractional ionization, and velocity distributions of the plume of ejecta which is typically encountered as the main desorption product in the mass spectrometry analysis of large biomolecules. Pulsed-ultraviolet (193 nm and 248 nm) desorption from 3-hydroxypicolinic acid (3-HPA) crystals was studied. Gas-phase absorption spectra of 3-HPA measured here revealed very different absorption cross-sections at these two wavelengths, and this was exploited to investigate the effects of plume absorption of the desorbing laser pulse. In both cases, LIF imaging revealed two plume components: a fast (maximum ∼ 0.1 cm / μs) low-intensity component which appear to be 3-HPA fragments, and a slower component of 3-HPA expanding at 0.05 cm / μs. In the case of ArF-laser irradiation, optical absorption spectroscopy indicated a breaking of the intramolecular hydrogen bond in the gas-phase matrix material.

KW - Laser

KW - Laser-induced fluorescence

KW - Plasma diagnostic technique

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SP - 248

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JO - Applied Surface Science

JF - Applied Surface Science

ER -

LIF imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes

Abstract

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Keywords

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