Leaving the Limits of Linearity for Light Microscopy

Nonlinear microscopy has enabled additional modalities for chemical contrast, deep penetration into biological tissues, and the ability to collect dynamics on ultrafast timescales across heterogeneous samples. The additional light fields introduced to a sample offer seemingly endless possibilities for variation to optimize and customize experimentation and the extraction of physical insight. This perspective highlights three areas of growth in this diverse field: the collection of information across multiple timescales, the selective imaging of interfacial chemistry, and the exploitation of quantum behavior for future imaging directions. Future innovations will leverage the work of the studies reviewed here as well as address the current challenges presented.