TY - JOUR
T1 - Laser-induced breakdown spectroscopy used to detect endophyte-mediated accumulation of metals by tall fescue
AU - Martin, Madhavi Z.
AU - Stewart, Arthur J.
AU - Gwinn, Kimberley D.
AU - Waller, John C.
PY - 2010/5/1
Y1 - 2010/5/1
N2 - Laser-induced breakdown spectroscopy (LIBS) was used to determine the impact of endophyte (Neotyphodium sp.) infection on elemental composition of tall fescue (Festuca arundinacea). Leaf material from endophyte-infected (E+) and endophyte-free (E-) tall fescue populations in established plots was examined. Leaf-tissue digestates were also tested for metals, by inductively coupled plasma (ICP) mass spectrometry (MS). Seven of eleven metals (Ca, Mg, Fe, Mn, Cu, Ni, and Zn) were measured by both techniques at concentrations great enough for a reliable comparison. Mg, Zn, and Cd, a toxic metal that can be present in forage, were readily detected by LIBS, even though Cd concentrations in the plants were below levels typically achieved using ICP MS detection. Implications of these results for research on forage analysis and phytoremediation are discussed.
AB - Laser-induced breakdown spectroscopy (LIBS) was used to determine the impact of endophyte (Neotyphodium sp.) infection on elemental composition of tall fescue (Festuca arundinacea). Leaf material from endophyte-infected (E+) and endophyte-free (E-) tall fescue populations in established plots was examined. Leaf-tissue digestates were also tested for metals, by inductively coupled plasma (ICP) mass spectrometry (MS). Seven of eleven metals (Ca, Mg, Fe, Mn, Cu, Ni, and Zn) were measured by both techniques at concentrations great enough for a reliable comparison. Mg, Zn, and Cd, a toxic metal that can be present in forage, were readily detected by LIBS, even though Cd concentrations in the plants were below levels typically achieved using ICP MS detection. Implications of these results for research on forage analysis and phytoremediation are discussed.
UR - http://www.scopus.com/inward/record.url?scp=77955959323&partnerID=8YFLogxK
U2 - 10.1364/AO.49.00C161
DO - 10.1364/AO.49.00C161
M3 - Article
AN - SCOPUS:77955959323
SN - 1559-128X
VL - 49
SP - C161-C167
JO - Applied Optics
JF - Applied Optics
IS - 13
ER -