Laser capture microdissection–liquid vortex capture mass spectrometry metabolic profiling of single onion epidermis and microalgae cells

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Abstract

Laser capture microdissection is a valuable technique in individually isolating single cells whether in tissue networks or deposited from a cell suspension. New developments have enabled coupling of laser capture microdissection with mass spectrometry via liquid vortex capture sampling probe. This enables online metabolic profiling of sectioned cells. Here, we describe the protocol used to deposit, isolate, and individually chemically characterize single Allium cepa and Chlamydomonas reinhardtii cells by laser capture microdissection–liquid vortex capture mass spectrometry.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages89-101
Number of pages13
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2064
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Funding

This work and J. F. C. was supported by the U.S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division and the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U.S. Department of Energy. V. K. was supported by the U. S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division.

FundersFunder number
UT-Battelle
U.S. Department of Energy
Office of Science
Basic Energy Sciences
Oak Ridge National Laboratory
Laboratory Directed Research and Development
Chemical Sciences, Geosciences, and Biosciences Division

    Keywords

    • Laser capture microdissection
    • Liquid-vortex capture
    • Mass spectrometry
    • Metabolites
    • Microalgae
    • Onion epidermis cell
    • Single cell

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