Laser capture microdissection–liquid vortex capture mass spectrometry metabolic profiling of single onion epidermis and microalgae cells

John F. Cahill; Vilmos Kertesz

doi:10.1007/978-1-4939-9831-9_7

Laser capture microdissection–liquid vortex capture mass spectrometry metabolic profiling of single onion epidermis and microalgae cells

John F. Cahill, Vilmos Kertesz

Bioanalytical Mass Spectrometry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

Laser capture microdissection is a valuable technique in individually isolating single cells whether in tissue networks or deposited from a cell suspension. New developments have enabled coupling of laser capture microdissection with mass spectrometry via liquid vortex capture sampling probe. This enables online metabolic profiling of sectioned cells. Here, we describe the protocol used to deposit, isolate, and individually chemically characterize single Allium cepa and Chlamydomonas reinhardtii cells by laser capture microdissection–liquid vortex capture mass spectrometry.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	89-101
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-4939-9831-9_7
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2064
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

This work and J. F. C. was supported by the U.S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division and the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U.S. Department of Energy. V. K. was supported by the U. S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division.

Keywords

Laser capture microdissection
Liquid-vortex capture
Mass spectrometry
Metabolites
Microalgae
Onion epidermis cell
Single cell

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10.1007/978-1-4939-9831-9_7

Cite this

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abstract = "Laser capture microdissection is a valuable technique in individually isolating single cells whether in tissue networks or deposited from a cell suspension. New developments have enabled coupling of laser capture microdissection with mass spectrometry via liquid vortex capture sampling probe. This enables online metabolic profiling of sectioned cells. Here, we describe the protocol used to deposit, isolate, and individually chemically characterize single Allium cepa and Chlamydomonas reinhardtii cells by laser capture microdissection–liquid vortex capture mass spectrometry.",

keywords = "Laser capture microdissection, Liquid-vortex capture, Mass spectrometry, Metabolites, Microalgae, Onion epidermis cell, Single cell",

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Laser capture microdissection–liquid vortex capture mass spectrometry metabolic profiling of single onion epidermis and microalgae cells. / Cahill, John F.; Kertesz, Vilmos.
Methods in Molecular Biology. Humana Press Inc., 2020. p. 89-101 (Methods in Molecular Biology; Vol. 2064).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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KW - Metabolites

KW - Microalgae

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Laser capture microdissection–liquid vortex capture mass spectrometry metabolic profiling of single onion epidermis and microalgae cells

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Keywords

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