Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum

Jessica L. Linville, Miguel Rodriguez, Jonathan R. Mielenz, Chris D. Cox

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a higher growth rate (μmax=1.223 vs. 0.571h-1) and less inhibition (KI,gen=0.991 vs. 0.757) in 10% v/v Populus hydrolysate compared to the WT. In 17.5% v/v Populus hydrolysate inhibition of PM increased slightly (KI,gen=0.888), whereas the WT was strongly inhibited and did not grow in a reproducible manner. Of the individual inhibitors tested, 4-hydroxybenzoic acid was the most inhibitory, followed by galacturonic acid. The PM did not have a greater ability to detoxify the hydrolysate than the WT.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalBioresource Technology
Volume147
DOIs
StatePublished - Nov 2013

Funding

This research was supported by the BioEnergy Science Center, a Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the Department of Energy Office of Science. Additional support was provided by the Institute for a Secure and Sustainable Environment at the University of Tennessee. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the DOE under Contract DE-AC05-00OR22725. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Keywords

  • Biofuels
  • Clostridium thermocellum
  • Inhibition
  • Kinetic model
  • Populus hydrolysate

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