Isolation, characterization, and pathogenicity of two pseudomonas syringae pathovars from populus trichocarpa seeds

Patricia M.B. Saint-Vincent; Mary Ridout; Nancy L. Engle; Travis J. Lawrence; Meredith L. Yeary; Timothy J. Tschaplinski; George Newcombe; Dale A. Pelletier

doi:10.3390/microorganisms8081137

Isolation, characterization, and pathogenicity of two pseudomonas syringae pathovars from populus trichocarpa seeds

Patricia M.B. Saint-Vincent, Mary Ridout, Nancy L. Engle, Travis J. Lawrence, Meredith L. Yeary, Timothy J. Tschaplinski, George Newcombe, Dale A. Pelletier

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populus trichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.

Original language	English
Article number	1137
Pages (from-to)	1-20
Number of pages	20
Journal	Microorganisms
Volume	8
Issue number	8
DOIs	https://doi.org/10.3390/microorganisms8081137
State	Published - Aug 2020

Funding

Funding: Part of this research was funded by the U.S. DOE Office of Biological and Environmental Research, Genomic Science Program as part of the Plant Microbe Interfaces Scientific Focus Area (http://pmi.ornl.gov) and Plant Feedstock Genomics, under Grant No. DE-SC0014547 Oak Ridge National Laboratory, UT-Battelle, LLC, for the US Department of Energy, Contract No. DEAC05-00OR22725 US Department of Energy Joint Genome Institute, Contract No. DE-AC02-05CH11231 and CSP1429 This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the US Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). Part of this research was funded by the U.S. DOE Office of Biological and Environmental Research, Genomic Science Program as part of the Plant Microbe Interfaces Scientific Focus Area (http://pmi.ornl.gov) and Plant Feedstock Genomics, under Grant No. DE-SC0014547 Oak Ridge National Laboratory, UT-Battelle, LLC, for the US Department of Energy, Contract No. DEAC05-00OR22725 US Department of Energy Joint Genome Institute, Contract No. DE-AC02-05CH11231 and CSP1429 This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the US Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). Acknowledgments: Special thanks to Mallory Ladd and Bob Hettich for assistance in running HR-LC-MS/MS samples, and to Tse-Yuan Lu for purifying genomic DNA for sequencing. Sara Jawdy provided leaves from greenhouse plants. Leah Burdick for growth assays and maintaining strain stocks. The DOE JGI CSP for genome sequence data.

Funders	Funder number
DOE Public Access Plan
Plant Microbe Interfaces Scientific Focus Area	DE-SC0014547
U.S. DOE Office of Biological and Environmental Research
US Department of Energy Joint Genome Institute	DE-AC05-00OR22725, DE-AC02-05CH11231, CSP1429
United States Government
U.S. Department of Energy	DEAC05-00OR22725
Oak Ridge National Laboratory
UT-Battelle

Keywords

Germination
Metabolomics
Pathogen
Pseudomonas
Virulence

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10.3390/microorganisms8081137

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title = "Isolation, characterization, and pathogenicity of two pseudomonas syringae pathovars from populus trichocarpa seeds",

abstract = "Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populus trichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.",

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author = "Saint-Vincent, {Patricia M.B.} and Mary Ridout and Engle, {Nancy L.} and Lawrence, {Travis J.} and Yeary, {Meredith L.} and Tschaplinski, {Timothy J.} and George Newcombe and Pelletier, {Dale A.}",

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language = "English",

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AU - Lawrence, Travis J.

AU - Yeary, Meredith L.

AU - Tschaplinski, Timothy J.

AU - Newcombe, George

AU - Pelletier, Dale A.

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N2 - Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populus trichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.

AB - Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populus trichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.

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Isolation, characterization, and pathogenicity of two pseudomonas syringae pathovars from populus trichocarpa seeds

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