Integration of top-down and bottom-up MS for comprehensive proteome characterization

Robert Hettich; Nathan VerBerkmoes; Keiji Asano; Joshua Sharp

Integration of top-down and bottom-up MS for comprehensive proteome characterization

Robert Hettich, Nathan VerBerkmoes, Keiji Asano, Joshua Sharp

Bioanalytical Mass Spectrometry

Research output: Contribution to conference › Paper › peer-review

Abstract

Integration of top-down and bottom-up mass spectroscopy (MS) method for detection of proteolytic peptides from a complex protein sample was discussed. Bacterial and yeast cells were cultured to suitable levels and a soluble protein fraction was prepared by centrifuging and separating the non-soluble material. The bottom-up MS was accomplished with a Thermo Finnigan LCQ instrument while the top-down MS was conducted with a IonSpec 9.4 FTICR-MS instrument. Results show that the approach provides the most comprehensive coverage of not only protein identification and cataloging, but also the exact molecular form of the protein on the cell.

Original language	English
Pages	53-54
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Integration of top-down and bottom-up MS for comprehensive proteome characterization",

abstract = "Integration of top-down and bottom-up mass spectroscopy (MS) method for detection of proteolytic peptides from a complex protein sample was discussed. Bacterial and yeast cells were cultured to suitable levels and a soluble protein fraction was prepared by centrifuging and separating the non-soluble material. The bottom-up MS was accomplished with a Thermo Finnigan LCQ instrument while the top-down MS was conducted with a IonSpec 9.4 FTICR-MS instrument. Results show that the approach provides the most comprehensive coverage of not only protein identification and cataloging, but also the exact molecular form of the protein on the cell.",

author = "Robert Hettich and Nathan VerBerkmoes and Keiji Asano and Joshua Sharp",

year = "2002",

language = "English",

pages = "53--54",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - Integration of top-down and bottom-up MS for comprehensive proteome characterization

AU - Hettich, Robert

AU - VerBerkmoes, Nathan

AU - Asano, Keiji

AU - Sharp, Joshua

PY - 2002

Y1 - 2002

N2 - Integration of top-down and bottom-up mass spectroscopy (MS) method for detection of proteolytic peptides from a complex protein sample was discussed. Bacterial and yeast cells were cultured to suitable levels and a soluble protein fraction was prepared by centrifuging and separating the non-soluble material. The bottom-up MS was accomplished with a Thermo Finnigan LCQ instrument while the top-down MS was conducted with a IonSpec 9.4 FTICR-MS instrument. Results show that the approach provides the most comprehensive coverage of not only protein identification and cataloging, but also the exact molecular form of the protein on the cell.

AB - Integration of top-down and bottom-up mass spectroscopy (MS) method for detection of proteolytic peptides from a complex protein sample was discussed. Bacterial and yeast cells were cultured to suitable levels and a soluble protein fraction was prepared by centrifuging and separating the non-soluble material. The bottom-up MS was accomplished with a Thermo Finnigan LCQ instrument while the top-down MS was conducted with a IonSpec 9.4 FTICR-MS instrument. Results show that the approach provides the most comprehensive coverage of not only protein identification and cataloging, but also the exact molecular form of the protein on the cell.

UR - http://www.scopus.com/inward/record.url?scp=2442690661&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442690661

SP - 53

EP - 54

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Integration of top-down and bottom-up MS for comprehensive proteome characterization

Abstract

Conference

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