Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Chang Geun Yoo; Yongil Yang; Yunqiao Pu; Xianzhi Meng; Wellington Muchero; Kelsey L. Yee; Olivia A. Thompson; Miguel Rodriguez; Garima Bali; Nancy L. Engle; Erika Lindquist; Vasanth Singan; Jeremy Schmutz; Stephen P. DiFazio; Timothy J. Tschaplinski; Gerald A. Tuskan; Jin Gui Chen; Brian Davison; Arthur J. Ragauskas

doi:10.1039/c7gc02219k

Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Chang Geun Yoo, Yongil Yang, Yunqiao Pu, Xianzhi Meng, Wellington Muchero, Kelsey L. Yee, Olivia A. Thompson, Miguel Rodriguez, Garima Bali, Nancy L. Engle, Erika Lindquist, Vasanth Singan, Jeremy Schmutz, Stephen P. DiFazio, Timothy J. Tschaplinski, Gerald A. Tuskan, Jin Gui Chen, Brian Davison, Arthur J. Ragauskas

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Abstract

Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

Original language	English
Pages (from-to)	5467-5478
Number of pages	12
Journal	Green Chemistry
Volume	19
Issue number	22
DOIs	https://doi.org/10.1039/c7gc02219k
State	Published - 2017

Funding

This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the pub- lisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).

Funders	Funder number
BioEnergy Science Center
DOE Office of Science
Department of Energy Bioenergy Research Center
Office of Biological and Environmental Research
U.S. Department of Energy
Office of Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c7gc02219k

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Yoo, C. G., Yang, Y., Pu, Y., Meng, X., Muchero, W., Yee, K. L., Thompson, O. A., Rodriguez, M., Bali, G., Engle, N. L., Lindquist, E., Singan, V., Schmutz, J., DiFazio, S. P., Tschaplinski, T. J., Tuskan, G. A., Chen, J. G., Davison, B., & Ragauskas, A. J. (2017). Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion. Green Chemistry, 19(22), 5467-5478. https://doi.org/10.1039/c7gc02219k

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title = "Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion",

abstract = "Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.",

author = "Yoo, {Chang Geun} and Yongil Yang and Yunqiao Pu and Xianzhi Meng and Wellington Muchero and Yee, {Kelsey L.} and Thompson, {Olivia A.} and Miguel Rodriguez and Garima Bali and Engle, {Nancy L.} and Erika Lindquist and Vasanth Singan and Jeremy Schmutz and DiFazio, {Stephen P.} and Tschaplinski, {Timothy J.} and Tuskan, {Gerald A.} and Chen, {Jin Gui} and Brian Davison and Ragauskas, {Arthur J.}",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2017.",

year = "2017",

doi = "10.1039/c7gc02219k",

language = "English",

volume = "19",

pages = "5467--5478",

journal = "Green Chemistry",

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Yoo, CG, Yang, Y, Pu, Y, Meng, X, Muchero, W, Yee, KL, Thompson, OA, Rodriguez, M, Bali, G, Engle, NL, Lindquist, E, Singan, V, Schmutz, J, DiFazio, SP, Tschaplinski, TJ , Tuskan, GA , Chen, JG , Davison, B & Ragauskas, AJ 2017, 'Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion', Green Chemistry, vol. 19, no. 22, pp. 5467-5478. https://doi.org/10.1039/c7gc02219k

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T1 - Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

AU - Yoo, Chang Geun

AU - Yang, Yongil

AU - Pu, Yunqiao

AU - Meng, Xianzhi

AU - Muchero, Wellington

AU - Yee, Kelsey L.

AU - Thompson, Olivia A.

AU - Rodriguez, Miguel

AU - Bali, Garima

AU - Engle, Nancy L.

AU - Lindquist, Erika

AU - Singan, Vasanth

AU - Schmutz, Jeremy

AU - DiFazio, Stephen P.

AU - Tschaplinski, Timothy J.

AU - Tuskan, Gerald A.

AU - Chen, Jin Gui

AU - Davison, Brian

AU - Ragauskas, Arthur J.

PY - 2017

Y1 - 2017

N2 - Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

AB - Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

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JO - Green Chemistry

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ER -

Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Abstract

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UN SDGs

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