TY - JOUR
T1 - Inhibition of the multidrug efflux pump LmrS from Staphylococcus aureus by cumin spice Cuminum cyminum
AU - Kakarla, Prathusha
AU - Floyd, Jared
AU - Mukherjee, Mun Mun
AU - Devireddy, Amith R.
AU - Inupakutika, Madhuri A.
AU - Ranweera, Indrika
AU - Kc, Ranjana
AU - Shrestha, Ugina
AU - Cheeti, Upender Rao
AU - Willmon, Thomas Mark
AU - Adams, Jaclyn
AU - Bruns, Merissa
AU - Gunda, Shravan Kumar
AU - Varela, Manuel F.
N1 - Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Staphylococcus aureus is a serious causative agent of infectious disease. Multidrug-resistant strains like methicillin-resistant S. aureus compromise treatment efficacy, causing significant morbidity and mortality. Active efflux represents a major antimicrobial resistance mechanism. The proton-driven multidrug efflux pump, LmrS, actively exports structurally distinct antimicrobials. To circumvent resistance and restore clinical efficacy of antibiotics, efflux pump inhibitors are necessary, and natural edible spices like cumin are potential candidates. The mode of cumin antibacterial action and underlying mechanisms behind drug resistance inhibition, however, are unclear. We tested the hypothesis that cumin inhibits LmrS drug transport. We found that cumin inhibited bacterial growth and LmrS ethidium transport in a dosage-dependent manner. We demonstrate that cumin is antibacterial toward a multidrug-resistant host and that resistance modulation involves multidrug efflux inhibition.
AB - Staphylococcus aureus is a serious causative agent of infectious disease. Multidrug-resistant strains like methicillin-resistant S. aureus compromise treatment efficacy, causing significant morbidity and mortality. Active efflux represents a major antimicrobial resistance mechanism. The proton-driven multidrug efflux pump, LmrS, actively exports structurally distinct antimicrobials. To circumvent resistance and restore clinical efficacy of antibiotics, efflux pump inhibitors are necessary, and natural edible spices like cumin are potential candidates. The mode of cumin antibacterial action and underlying mechanisms behind drug resistance inhibition, however, are unclear. We tested the hypothesis that cumin inhibits LmrS drug transport. We found that cumin inhibited bacterial growth and LmrS ethidium transport in a dosage-dependent manner. We demonstrate that cumin is antibacterial toward a multidrug-resistant host and that resistance modulation involves multidrug efflux inhibition.
KW - Cumin
KW - Dietary spice
KW - Efflux
KW - Efflux pump inhibitor
KW - LmrS
KW - MRSA
KW - Multidrug resistance
KW - Natural antimicrobials
KW - Spices
KW - Staphylococcus aureus
UR - https://www.scopus.com/pages/publications/84994473948
U2 - 10.1007/s00203-016-1314-5
DO - 10.1007/s00203-016-1314-5
M3 - Article
C2 - 27830269
AN - SCOPUS:84994473948
SN - 0302-8933
VL - 199
SP - 465
EP - 474
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 3
ER -