Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

G. A. Tuskan; W. A. Sargent; T. Rensema; J. A. Walla

doi:10.1007/BF00034756

Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

G. A. Tuskan, W. A. Sargent, T. Rensema, J. A. Walla

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Abstract

Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 μM BA:5.4 μM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0 μM BA: 5.4 μM NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0 μM BA: 0.054 μM NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis.

Original language	English
Pages (from-to)	47-52
Number of pages	6
Journal	Plant Cell, Tissue and Organ Culture
Volume	20
Issue number	1
DOIs	https://doi.org/10.1007/BF00034756
State	Published - Jan 1990

Keywords

callus culture
caulogenesis
in vitro screening
ponderosa pine
western gall rust

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title = "Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants",

abstract = "Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 μM BA:5.4 μM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0 μM BA: 5.4 μM NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0 μM BA: 0.054 μM NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis.",

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AU - Sargent, W. A.

AU - Rensema, T.

AU - Walla, J. A.

PY - 1990/1

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N2 - Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 μM BA:5.4 μM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0 μM BA: 5.4 μM NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0 μM BA: 0.054 μM NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis.

AB - Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 μM BA:5.4 μM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0 μM BA: 5.4 μM NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0 μM BA: 0.054 μM NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis.

KW - callus culture

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KW - western gall rust

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Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

Abstract

Keywords

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