Inference of photosynthetic capacity parameters from chlorophyll a fluorescence is affected by redox state of PSII reaction centers

Solar-induced chlorophyll fluorescence (SIF) has been used to infer photosynthetic capacity parameters (e.g., the maximum carboxylation rate V_cmax, and the maximum electron transport rate J_max). However, the precise mechanism and practical utility of such approach under dynamic environments remain unclear. We used the balance between the light and carbon reactions to derive theoretical equations relating chlorophyll a fluorescence (ChlF) emission and photosynthetic capacity parameters, and formulated testable hypotheses regarding the dynamic relationships between the true total ChlF emitted from PSII (SIF_PSII) and V_cmax and J_max. We employed concurrent measurements of gas exchanges and ChlF parameters for 15 species from six biomes to test the formulated hypotheses across species, temperatures, and limitation state of carboxylation. Our results revealed that SIF_PSII alone is incapable of informing the variations in V_cmax and J_max across species, even when SIF_PSII is determined under the same environmental conditions. In contrast, the product of SIF_PSII and the fraction of open PSII reactions q_L, which indicates the redox state of PSII, is a strong predictor of both V_cmax and J_max, although their precise relationships vary somewhat with environmental conditions. Our findings suggest the redox state of PSII strongly influences the relationship between SIF_PSII and V_cmax and J_max.