Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces

István Bakos; Ádám Vass; Eric S. Muckley; Ilia N. Ivanov; Zsófia Keresztes

doi:10.1016/j.elecom.2021.106961

Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces

István Bakos, Ádám Vass, Eric S. Muckley, Ilia N. Ivanov, Zsófia Keresztes

Functional Hybrid Nanomaterials

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We demonstrate the use of copper underpotential deposition (UPD) for measurement of the electrochemically active surface area of a protein-modified gold electrode. The kinetics of adsorption and the subsequent structural rearrangement of β-casein, as a model protein, on the surface of a gold electrode have been followed over time. The protein-free surface area was determined by measuring the charge derived from UPD copper deposition on the non-blocked surface area at different stages of adsorption. The behaviour of β-casein adsorption as a function of surface roughness and the microstructure of the gold electrode, as well as the concentration of the protein solution, are studied using this method.

Original language	English
Article number	106961
Journal	Electrochemistry Communications
Volume	124
DOIs	https://doi.org/10.1016/j.elecom.2021.106961
State	Published - Mar 2021

Funding

The authors thankfully acknowledge the financial support of 690898/H2020-MSCA-RISE-2015 “FORMILK” “Innovative technology for the detection of enzyme activity in milk” and BIONANO_GINOP-2.3.2-15-2016-00017 projects. This research was partially conducted at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility.

Keywords

Copper underpotential deposition
Protein adsorption
Surface coverage

Access to Document

10.1016/j.elecom.2021.106961

Cite this

@article{e9ccd546f59c49b8bd709187ede843f1,

title = "Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces",

abstract = "We demonstrate the use of copper underpotential deposition (UPD) for measurement of the electrochemically active surface area of a protein-modified gold electrode. The kinetics of adsorption and the subsequent structural rearrangement of β-casein, as a model protein, on the surface of a gold electrode have been followed over time. The protein-free surface area was determined by measuring the charge derived from UPD copper deposition on the non-blocked surface area at different stages of adsorption. The behaviour of β-casein adsorption as a function of surface roughness and the microstructure of the gold electrode, as well as the concentration of the protein solution, are studied using this method.",

keywords = "Copper underpotential deposition, Protein adsorption, Surface coverage",

author = "Istv{\'a}n Bakos and {\'A}d{\'a}m Vass and Muckley, {Eric S.} and Ivanov, {Ilia N.} and Zs{\'o}fia Keresztes",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = mar,

doi = "10.1016/j.elecom.2021.106961",

language = "English",

volume = "124",

journal = "Electrochemistry Communications",

issn = "1388-2481",

publisher = "Elsevier",

}

TY - JOUR

T1 - Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces

AU - Bakos, István

AU - Vass, Ádám

AU - Muckley, Eric S.

AU - Ivanov, Ilia N.

AU - Keresztes, Zsófia

PY - 2021/3

Y1 - 2021/3

N2 - We demonstrate the use of copper underpotential deposition (UPD) for measurement of the electrochemically active surface area of a protein-modified gold electrode. The kinetics of adsorption and the subsequent structural rearrangement of β-casein, as a model protein, on the surface of a gold electrode have been followed over time. The protein-free surface area was determined by measuring the charge derived from UPD copper deposition on the non-blocked surface area at different stages of adsorption. The behaviour of β-casein adsorption as a function of surface roughness and the microstructure of the gold electrode, as well as the concentration of the protein solution, are studied using this method.

AB - We demonstrate the use of copper underpotential deposition (UPD) for measurement of the electrochemically active surface area of a protein-modified gold electrode. The kinetics of adsorption and the subsequent structural rearrangement of β-casein, as a model protein, on the surface of a gold electrode have been followed over time. The protein-free surface area was determined by measuring the charge derived from UPD copper deposition on the non-blocked surface area at different stages of adsorption. The behaviour of β-casein adsorption as a function of surface roughness and the microstructure of the gold electrode, as well as the concentration of the protein solution, are studied using this method.

KW - Copper underpotential deposition

KW - Protein adsorption

KW - Surface coverage

UR - http://www.scopus.com/inward/record.url?scp=85100763789&partnerID=8YFLogxK

U2 - 10.1016/j.elecom.2021.106961

DO - 10.1016/j.elecom.2021.106961

M3 - Article

AN - SCOPUS:85100763789

SN - 1388-2481

VL - 124

JO - Electrochemistry Communications

JF - Electrochemistry Communications

M1 - 106961

ER -

Indirect electrochemical method for high accuracy quantification of protein adsorption on gold surfaces

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this