In Vivo Mercury (De)Methylation Metabolism in Cephalopods under Different pCO2 Scenarios

Sophie Gentès; Antoine Minet; Christelle Lopes; Emmanuel Tessier; Claire Gassie; Rémy Guyoneaud; Peter W. Swarzenski; Paco Bustamante; Marc Metian; David Amouroux; Thomas Lacoue-Labarthe

doi:10.1021/acs.est.2c08513

In Vivo Mercury (De)Methylation Metabolism in Cephalopods under Different pCO₂ Scenarios

Sophie Gentès, Antoine Minet, Christelle Lopes, Emmanuel Tessier, Claire Gassie, Rémy Guyoneaud, Peter W. Swarzenski, Paco Bustamante, Marc Metian, David Amouroux, Thomas Lacoue-Labarthe

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This work quantified the accumulation efficiencies of Hg in cuttlefish, depending on both organic (MeHg) and inorganic (Hg(II)) forms, under increased pCO₂ (1600 μatm). Cuttlefish were fed with live shrimps injected with two Hg stable isotopic tracers (Me²⁰²Hg and ¹⁹⁹Hg(II)), which allowed for the simultaneous quantification of internal Hg accumulation, Hg(II) methylation, and MeHg demethylation rates in different organs. Results showed that pCO₂ had no impact on Hg bioaccumulation and organotropism, and both Hg and pCO₂ did not influence the microbiota diversity of gut and digestive gland. However, the results also demonstrated that the digestive gland is a key organ for in vivo MeHg demethylation. Consequently, cuttlefish exposed to environmental levels of MeHg could exhibit in vivo MeHg demethylation. We hypothesize that in vivo MeHg demethylation could be due to biologically induced reactions or to abiotic reactions. This has important implications as to how some marine organisms may respond to future ocean change and global mercury contamination.

Original language	English
Pages (from-to)	5761-5770
Number of pages	10
Journal	Environmental Science and Technology
Volume	57
Issue number	14
DOIs	https://doi.org/10.1021/acs.est.2c08513
State	Published - Apr 11 2023
Externally published	Yes

Funding

The authors acknowledge technical support provided by the IAEA Monaco-based marine laboratories. The IAEA is grateful for the support provided to its Marine Environment Laboratories by the Government of the Principality of Monaco. The authors thank François Oberhänsli and Angus Taylor for their help for cuttlefish maintenance. This work is a contribution to the MERCy project funded by la Fondation pour la Recherche sur la Biodiversité and the Ministère de la Transition Ecologique et Solidaire. The Région Nouvelle Aquitaine is acknowledged for its support to the post-doc and the PhD grants to S.G. and A.M., respectively, through the EXPO project (Exposition des prédateurs de la zone intertidale aux contaminants). This work was supported by the project ECONAT Axe 1 - Ressources Marines Littorales: qualité et éco-valorisation, funded by the Contrat de Plan Etat-Région and the CNRS and the European Regional Development Fund through the project QUALIDRIS (Ressources Marines et Littorales: Qualité et Eco-valorisation). The Institut Universitaire de France (IUF) is acknowledged for its support to PB as a Senior Member. This work benefitted from the French GDR ″Aquatic Ecotoxicology″ framework, which aims at fostering stimulating scientific discussions and collaborations for more integrative approaches. The authors warmly thank Antoine and José Lacoue-Labarthe, Elodie Réveillac, Jérôme Fort, and Fernando Pedraza for their support during field sampling. This contribution was made within the framework of the IAEA CRP on “Applied radioecological tracers to assess coastal and marine ecosystem health” (K41019). This work was supported by la Fondation pour la Recherche sur la Biodiversité and the Ministère de la Transition Ecologique et Solidaire through the MERCY project and by the Région Nouvelle-Aquitaine through the EXPO project. The authors acknowledge technical support provided by the IAEA Monaco-based marine laboratories. The IAEA is grateful for the support provided to its Marine Environment Laboratories by the Government of the Principality of Monaco. The authors thank François Oberhänsli and Angus Taylor for their help for cuttlefish maintenance. This work is a contribution to the MERCy project funded by la Fondation pour la Recherche sur la Biodiversité and the Ministère de la Transition Ecologique et Solidaire. The Région Nouvelle Aquitaine is acknowledged for its support to the post-doc and the PhD grants to S.G. and A.M., respectively, through the EXPO project (Exposition des prédateurs de la zone intertidale aux contaminants). This work was supported by the project ECONAT Axe 1 - Ressources Marines Littorales: qualité et éco-valorisation, funded by the Contrat de Plan Etat-Région and the CNRS and the European Regional Development Fund through the project QUALIDRIS (Ressources Marines et Littorales: Qualité et Eco-valorisation). The Institut Universitaire de France (IUF) is acknowledged for its support to PB as a Senior Member. This work benefitted from the French GDR ″Aquatic Ecotoxicology″ framework, which aims at fostering stimulating scientific discussions and collaborations for more integrative approaches. The authors warmly thank Antoine and José Lacoue-Labarthe, Elodie Réveillac, Jérôme Fort, and Fernando Pedraza for their support during field sampling. This contribution was made within the framework of the IAEA CRP on “Applied radioecological tracers to assess coastal and marine ecosystem health” (K41019).

Funders	Funder number
Contrat de Plan Etat-Région
Government of the Principality of Monaco
International Atomic Energy Agency
Centre National de la Recherche Scientifique
Institut universitaire de France	K41019
European Regional Development Fund
Ministère de la Transition écologique et Solidaire

Keywords

acidification
cuttlefish
enriched isotopes
inorganic mercury
methylmercury
microbiota

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/acs.est.2c08513

Cite this

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title = "In Vivo Mercury (De)Methylation Metabolism in Cephalopods under Different pCO2 Scenarios",

abstract = "This work quantified the accumulation efficiencies of Hg in cuttlefish, depending on both organic (MeHg) and inorganic (Hg(II)) forms, under increased pCO2 (1600 μatm). Cuttlefish were fed with live shrimps injected with two Hg stable isotopic tracers (Me202Hg and 199Hg(II)), which allowed for the simultaneous quantification of internal Hg accumulation, Hg(II) methylation, and MeHg demethylation rates in different organs. Results showed that pCO2 had no impact on Hg bioaccumulation and organotropism, and both Hg and pCO2 did not influence the microbiota diversity of gut and digestive gland. However, the results also demonstrated that the digestive gland is a key organ for in vivo MeHg demethylation. Consequently, cuttlefish exposed to environmental levels of MeHg could exhibit in vivo MeHg demethylation. We hypothesize that in vivo MeHg demethylation could be due to biologically induced reactions or to abiotic reactions. This has important implications as to how some marine organisms may respond to future ocean change and global mercury contamination.",

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AU - Minet, Antoine

AU - Lopes, Christelle

AU - Tessier, Emmanuel

AU - Gassie, Claire

AU - Guyoneaud, Rémy

AU - Swarzenski, Peter W.

AU - Bustamante, Paco

AU - Metian, Marc

AU - Amouroux, David

AU - Lacoue-Labarthe, Thomas

PY - 2023/4/11

Y1 - 2023/4/11

N2 - This work quantified the accumulation efficiencies of Hg in cuttlefish, depending on both organic (MeHg) and inorganic (Hg(II)) forms, under increased pCO2 (1600 μatm). Cuttlefish were fed with live shrimps injected with two Hg stable isotopic tracers (Me202Hg and 199Hg(II)), which allowed for the simultaneous quantification of internal Hg accumulation, Hg(II) methylation, and MeHg demethylation rates in different organs. Results showed that pCO2 had no impact on Hg bioaccumulation and organotropism, and both Hg and pCO2 did not influence the microbiota diversity of gut and digestive gland. However, the results also demonstrated that the digestive gland is a key organ for in vivo MeHg demethylation. Consequently, cuttlefish exposed to environmental levels of MeHg could exhibit in vivo MeHg demethylation. We hypothesize that in vivo MeHg demethylation could be due to biologically induced reactions or to abiotic reactions. This has important implications as to how some marine organisms may respond to future ocean change and global mercury contamination.

AB - This work quantified the accumulation efficiencies of Hg in cuttlefish, depending on both organic (MeHg) and inorganic (Hg(II)) forms, under increased pCO2 (1600 μatm). Cuttlefish were fed with live shrimps injected with two Hg stable isotopic tracers (Me202Hg and 199Hg(II)), which allowed for the simultaneous quantification of internal Hg accumulation, Hg(II) methylation, and MeHg demethylation rates in different organs. Results showed that pCO2 had no impact on Hg bioaccumulation and organotropism, and both Hg and pCO2 did not influence the microbiota diversity of gut and digestive gland. However, the results also demonstrated that the digestive gland is a key organ for in vivo MeHg demethylation. Consequently, cuttlefish exposed to environmental levels of MeHg could exhibit in vivo MeHg demethylation. We hypothesize that in vivo MeHg demethylation could be due to biologically induced reactions or to abiotic reactions. This has important implications as to how some marine organisms may respond to future ocean change and global mercury contamination.

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