In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets

R. A. Hill; G. A. Tuskan; A. A. Boe

doi:10.1007/BF00042283

In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets

R. A. Hill, G. A. Tuskan, A. A. Boe

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Abstract

Ovaries from immature florets were selected as an improved explant source for in vitro cultures of Hosta sieboldiana. Culturing for 30 days on a modified Murashige & Skoog (MS) medium supplemented with thiamine (0.4 mgl^-1) and glycine (2.0 mgl^-1) induced the highest frequency of callus growth at 5.4 μM NAA and 4.4 μM BA, and the highest frequency of shoot initiation at 0.4 μM BA. The severed basal portion of the ovary was the site of shoot growth. Eliminating phytohormones and adding 80 mgl^-1 of AdSO₄ significantly enhanced root initiation. Rooted plantlets were established in sterilized vermiculite containing MS mineral salts. A cold treatment (1°C) of 30 days in the dark was necessary to break dormancy of the rooted plantlets.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	Plant Cell, Tissue and Organ Culture
Volume	17
Issue number	1
DOIs	https://doi.org/10.1007/BF00042283
State	Published - Apr 1989

Keywords

adenine sulfate
hosta
micropropagation
organogenesis

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10.1007/BF00042283

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title = "In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets",

abstract = "Ovaries from immature florets were selected as an improved explant source for in vitro cultures of Hosta sieboldiana. Culturing for 30 days on a modified Murashige & Skoog (MS) medium supplemented with thiamine (0.4 mgl-1) and glycine (2.0 mgl-1) induced the highest frequency of callus growth at 5.4 μM NAA and 4.4 μM BA, and the highest frequency of shoot initiation at 0.4 μM BA. The severed basal portion of the ovary was the site of shoot growth. Eliminating phytohormones and adding 80 mgl-1 of AdSO4 significantly enhanced root initiation. Rooted plantlets were established in sterilized vermiculite containing MS mineral salts. A cold treatment (1°C) of 30 days in the dark was necessary to break dormancy of the rooted plantlets.",

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T1 - In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets

AU - Hill, R. A.

AU - Tuskan, G. A.

AU - Boe, A. A.

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N2 - Ovaries from immature florets were selected as an improved explant source for in vitro cultures of Hosta sieboldiana. Culturing for 30 days on a modified Murashige & Skoog (MS) medium supplemented with thiamine (0.4 mgl-1) and glycine (2.0 mgl-1) induced the highest frequency of callus growth at 5.4 μM NAA and 4.4 μM BA, and the highest frequency of shoot initiation at 0.4 μM BA. The severed basal portion of the ovary was the site of shoot growth. Eliminating phytohormones and adding 80 mgl-1 of AdSO4 significantly enhanced root initiation. Rooted plantlets were established in sterilized vermiculite containing MS mineral salts. A cold treatment (1°C) of 30 days in the dark was necessary to break dormancy of the rooted plantlets.

AB - Ovaries from immature florets were selected as an improved explant source for in vitro cultures of Hosta sieboldiana. Culturing for 30 days on a modified Murashige & Skoog (MS) medium supplemented with thiamine (0.4 mgl-1) and glycine (2.0 mgl-1) induced the highest frequency of callus growth at 5.4 μM NAA and 4.4 μM BA, and the highest frequency of shoot initiation at 0.4 μM BA. The severed basal portion of the ovary was the site of shoot growth. Eliminating phytohormones and adding 80 mgl-1 of AdSO4 significantly enhanced root initiation. Rooted plantlets were established in sterilized vermiculite containing MS mineral salts. A cold treatment (1°C) of 30 days in the dark was necessary to break dormancy of the rooted plantlets.

KW - adenine sulfate

KW - hosta

KW - micropropagation

KW - organogenesis

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In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets

Abstract

Keywords

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