In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Alex Belianinov; Alison Pawlicki; Matt Burch; Songkil Kim; Anton Ievlev; Jeff Fowler; Olga Ovchinnikova

doi:10.1116/1.5040849

In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Alex Belianinov, Alison Pawlicki, Matt Burch, Songkil Kim, Anton Ievlev, Jeff Fowler, Olga Ovchinnikova

Functional Atomic Force Microscopy Group

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He⁺ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.

Original language	English
Article number	051803
Journal	Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics
Volume	36
Issue number	5
DOIs	https://doi.org/10.1116/1.5040849
State	Published - Sep 1 2018

Funding

This paper has been authored by UT-Battelle, LLC, under Contract No. DE-AC0500OR22725 with the U.S. Department of Energy. The United States Government retains, and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a nonexclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for the United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). The HIM imaging, image analytics, and simulations portion of this research were conducted at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility. A.P. and J.F. were supported by Syngenta Crop Protection.

Funders	Funder number
Syngenta Crop Protection

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10.1116/1.5040849

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title = "In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy",

abstract = "Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He+ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.",

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AU - Pawlicki, Alison

AU - Burch, Matt

AU - Kim, Songkil

AU - Ievlev, Anton

AU - Fowler, Jeff

AU - Ovchinnikova, Olga

PY - 2018/9/1

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AB - Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He+ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.

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In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

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