In situ characterization of boehmite particles in water using liquid sem

Juan Yao; Bruce W. Arey; Li Yang; Fei Zhang; Rachel Komorek; Jaehun Chun; Xiao Ying Yu

doi:10.3791/56058

In situ characterization of boehmite particles in water using liquid sem

Juan Yao, Bruce W. Arey, Li Yang, Fei Zhang, Rachel Komorek, Jaehun Chun, Xiao Ying Yu

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface (SALVI) and Scanning Electron Microscopy (SEM). This paper describes the method and key steps in integrating the vacuum compatible SAVLI to SEM and obtaining secondary electron (SE) images of particles in liquid in high vacuum. Energy dispersive x-ray spectroscopy (EDX) is used to obtain elemental analysis of particles in liquid and control samples including deionized (DI) water only and an empty channel as well. Synthesized boehmite (AlOOH) particles suspended in liquid are used as a model in the liquid SEM illustration. The results demonstrate that the particles can be imaged in the SE mode with good resolution (i.e., 400 nm). The AlOOH EDX spectrum shows significant signal from the aluminum (Al) when compared with the DI water and the empty channel control. In situ liquid SEM is a powerful technique to study particles in liquid with many exciting applications. This procedure aims to provide technical know-how in order to conduct liquid SEM imaging and EDX analysis using SALVI and to reduce potential pitfalls when using this approach.

Original language	English
Article number	e56058
Journal	Journal of Visualized Experiments
Volume	2017
Issue number	127
DOIs	https://doi.org/10.3791/56058
State	Published - Sep 27 2017
Externally published	Yes

Funding

We are grateful to the Pacific Northwest National Laboratory (PNNL) Nuclear Process Science Initiative (NPSI)-Laboratory Directed Research and Development (LDRD) fund for support. Dr. Sayandev Chatterjee provided the synthesized boehmite particles. Instrumental access was provided through a W. R. Wiley Environmental Molecular Sciences Laboratory (EMSL) General User Proposal. EMSL is a national scientific user facility sponsored by the Office of Biological and Environmental Research (BER) at PNNL. PNNL is operated by Battelle for the DOE under Contract DE-AC05-76RL01830.

Keywords

Boehmite
Chemistry
Elemental composition mapping
Imaging
In situ liquid scanning electron microscopy
Issue 127
Microfluidics
Scanning electron microscopy

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title = "In situ characterization of boehmite particles in water using liquid sem",

abstract = "In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface (SALVI) and Scanning Electron Microscopy (SEM). This paper describes the method and key steps in integrating the vacuum compatible SAVLI to SEM and obtaining secondary electron (SE) images of particles in liquid in high vacuum. Energy dispersive x-ray spectroscopy (EDX) is used to obtain elemental analysis of particles in liquid and control samples including deionized (DI) water only and an empty channel as well. Synthesized boehmite (AlOOH) particles suspended in liquid are used as a model in the liquid SEM illustration. The results demonstrate that the particles can be imaged in the SE mode with good resolution (i.e., 400 nm). The AlOOH EDX spectrum shows significant signal from the aluminum (Al) when compared with the DI water and the empty channel control. In situ liquid SEM is a powerful technique to study particles in liquid with many exciting applications. This procedure aims to provide technical know-how in order to conduct liquid SEM imaging and EDX analysis using SALVI and to reduce potential pitfalls when using this approach.",

keywords = "Boehmite, Chemistry, Elemental composition mapping, Imaging, In situ liquid scanning electron microscopy, Issue 127, Microfluidics, Scanning electron microscopy",

author = "Juan Yao and Arey, {Bruce W.} and Li Yang and Fei Zhang and Rachel Komorek and Jaehun Chun and Yu, {Xiao Ying}",

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AU - Arey, Bruce W.

AU - Yang, Li

AU - Zhang, Fei

AU - Komorek, Rachel

AU - Chun, Jaehun

AU - Yu, Xiao Ying

PY - 2017/9/27

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AB - In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface (SALVI) and Scanning Electron Microscopy (SEM). This paper describes the method and key steps in integrating the vacuum compatible SAVLI to SEM and obtaining secondary electron (SE) images of particles in liquid in high vacuum. Energy dispersive x-ray spectroscopy (EDX) is used to obtain elemental analysis of particles in liquid and control samples including deionized (DI) water only and an empty channel as well. Synthesized boehmite (AlOOH) particles suspended in liquid are used as a model in the liquid SEM illustration. The results demonstrate that the particles can be imaged in the SE mode with good resolution (i.e., 400 nm). The AlOOH EDX spectrum shows significant signal from the aluminum (Al) when compared with the DI water and the empty channel control. In situ liquid SEM is a powerful technique to study particles in liquid with many exciting applications. This procedure aims to provide technical know-how in order to conduct liquid SEM imaging and EDX analysis using SALVI and to reduce potential pitfalls when using this approach.

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KW - Scanning electron microscopy

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In situ characterization of boehmite particles in water using liquid sem

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