TY - JOUR
T1 - Improvement of oligonucleotide probe design criteria for functional gene microarrays in environmental applications
AU - Liebich, Jost
AU - Schadt, Christopher W.
AU - Chong, Song C.
AU - He, Zhili
AU - Rhee, Sung Keun
AU - Zhou, Jizhong
PY - 2006/2
Y1 - 2006/2
N2 - To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (≤90%), the length of identical sequence stretches (≤20 bases), and the binding free energy (≥-35 kcal mol-1) was found to be predictive of probe specificity.
AB - To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (≤90%), the length of identical sequence stretches (≤20 bases), and the binding free energy (≥-35 kcal mol-1) was found to be predictive of probe specificity.
UR - http://www.scopus.com/inward/record.url?scp=33144457149&partnerID=8YFLogxK
U2 - 10.1128/AEM.72.2.1688-1691.2006
DO - 10.1128/AEM.72.2.1688-1691.2006
M3 - Article
C2 - 16461729
AN - SCOPUS:33144457149
SN - 0099-2240
VL - 72
SP - 1688
EP - 1691
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 2
ER -