Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wetdiskmilling

Akio Kumagai; Long Wu; Shinichiro Iwamoto; Seung Hwan Lee; Takashi Endo; Miguel Rodriguez; Jonathan R. Mielenz

doi:10.1016/j.renene.2014.11.070

Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wetdiskmilling

Akio Kumagai, Long Wu, Shinichiro Iwamoto, Seung Hwan Lee, Takashi Endo, Miguel Rodriguez, Jonathan R. Mielenz

Biomaterials and Biomass Characterizatio

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150°C for 60min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (. Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150°C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.

Original language	English
Pages (from-to)	782-789
Number of pages	8
Journal	Renewable Energy
Volume	76
DOIs	https://doi.org/10.1016/j.renene.2014.11.070
State	Published - Apr 1 2015

Keywords

Biorefinery
Hot-compressed water
Populus
Switchgrass
Wet disk milling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.renene.2014.11.070

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title = "Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wetdiskmilling",

abstract = "To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150°C for 60min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (. Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150°C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.",

keywords = "Biorefinery, Hot-compressed water, Populus, Switchgrass, Wet disk milling",

author = "Akio Kumagai and Long Wu and Shinichiro Iwamoto and Lee, {Seung Hwan} and Takashi Endo and Miguel Rodriguez and Mielenz, {Jonathan R.}",

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doi = "10.1016/j.renene.2014.11.070",

language = "English",

volume = "76",

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journal = "Renewable Energy",

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TY - JOUR

T1 - Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wetdiskmilling

AU - Kumagai, Akio

AU - Wu, Long

AU - Iwamoto, Shinichiro

AU - Lee, Seung Hwan

AU - Endo, Takashi

AU - Rodriguez, Miguel

AU - Mielenz, Jonathan R.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150°C for 60min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (. Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150°C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.

AB - To reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST; 130 and 150°C for 60min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: Populus trichocarpa and switchgrass (. Panicum virgatum). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. After steam treatment at 150°C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.

KW - Biorefinery

KW - Hot-compressed water

KW - Populus

KW - Switchgrass

KW - Wet disk milling

UR - http://www.scopus.com/inward/record.url?scp=84919897666&partnerID=8YFLogxK

U2 - 10.1016/j.renene.2014.11.070

DO - 10.1016/j.renene.2014.11.070

M3 - Article

AN - SCOPUS:84919897666

SN - 0960-1481

VL - 76

SP - 782

EP - 789

JO - Renewable Energy

JF - Renewable Energy

ER -

Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wetdiskmilling

Abstract

Keywords

UN SDGs

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