Immunological approaches to plant cell wall and biomass characterization: Glycome profiling

Sivakumar Pattathil, Utku Avci, Jeffrey S. Miller, Michael G. Hahn

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

137 Scopus citations

Abstract

The native complexity of plant cell walls makes research on them challenging. Hence, it is advantageous to have a diversity of tools that can be used to analyze and characterize plant cell walls. In this chapter, we describe one of two immunological approaches that can be employed for screening of plant cell wall/biomass materials from diverse plants and tissues. This approach, Glycome Profiling, lends itself well to moderate to high-throughput screening of plant cell wall/biomass samples. Glycome Profiling is being further optimized to reduce the amount of sample required for the analysis, and to improve the sensitivity and throughput of the assay. We are optimistic that Glycome Profiling will prove to be a broadly applicable experimental approach that will find increasing application to a wide variety of studies on plant cell wall/biomass samples.

Original languageEnglish
Title of host publicationBiomass Conversion
Subtitle of host publicationMethods and Protocols
EditorsMichael Himmel
Pages61-72
Number of pages12
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume908
ISSN (Print)1064-3745

Keywords

  • Biomass
  • Cell wall glycans
  • ELISA
  • Glycome profiling
  • Heatmap
  • Monoclonal antibody

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