Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H2O2 sensing

Kang Deuk Seo; Seong Dae Oh; Seong Ho Choi; Sang Hyub Oh; Jin Chun Woo; Sang Ho Kim

doi:10.4028/0-87849-436-7.869

Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H₂O₂ sensing

Kang Deuk Seo
, Seong Dae Oh
, Seong Ho Choi
, Sang Hyub Oh
, Jin Chun Woo
, Sang Ho Kim

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANI-MWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H₂O₂ was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H₂O₂ was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.

Original language	English
Pages (from-to)	869-872
Number of pages	4
Journal	Key Engineering Materials
Volume	342-343
DOIs	https://doi.org/10.4028/0-87849-436-7.869
State	Published - 2007
Externally published	Yes

Keywords

Horseradish peroxide
Immobilization Covalent immobilization
Physical immobilization
Self-assembling

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10.4028/0-87849-436-7.869

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title = "Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H2O2 sensing",

abstract = "In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANI-MWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.",

keywords = "Horseradish peroxide, Immobilization Covalent immobilization, Physical immobilization, Self-assembling",

author = "Seo, \{Kang Deuk\} and Oh, \{Seong Dae\} and Choi, \{Seong Ho\} and Oh, \{Sang Hyub\} and Woo, \{Jin Chun\} and Kim, \{Sang Ho\}",

year = "2007",

doi = "10.4028/0-87849-436-7.869",

language = "English",

volume = "342-343",

pages = "869--872",

journal = "Key Engineering Materials",

issn = "1013-9826",

publisher = "Trans Tech Publications",

}

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T1 - Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H2O2 sensing

AU - Seo, Kang Deuk

AU - Oh, Seong Dae

AU - Choi, Seong Ho

AU - Oh, Sang Hyub

AU - Woo, Jin Chun

AU - Kim, Sang Ho

PY - 2007

Y1 - 2007

N2 - In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANI-MWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.

AB - In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANI-MWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.

KW - Horseradish peroxide

KW - Immobilization Covalent immobilization

KW - Physical immobilization

KW - Self-assembling

UR - https://www.scopus.com/pages/publications/34147092721

U2 - 10.4028/0-87849-436-7.869

DO - 10.4028/0-87849-436-7.869

M3 - Article

AN - SCOPUS:34147092721

SN - 1013-9826

VL - 342-343

SP - 869

EP - 872

JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H₂O₂ sensing

Abstract

Keywords

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