Immobilization of cell adhesive RGD peptide onto the surface of highly porous biodegradable polymer scaffolds fabricated by a gas foaming/salt leaching method

Jun Jin Yoon, Soon Ho Song, Doo Sung Lee, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

139 Scopus citations

Abstract

A cell adhesive peptide moiety, Gly-Arg-Gly-Asp-Tyr (GRGDY), was immobilized onto the surface of highly porous biodegradable polymer scaffolds for enhancing cell adhesion and function. A carboxyl terminal end of poly(D,L-lactic-co-glycolic acid) (PLGA) was functionalized with a primary amine group by conjugating hexaethylene glycol-diamine. The PLGA-NH2 was blended with PLGA in varying ratios to prepare films by solvent casting or to fabricate porous scaffolds by a gas foaming/salt leaching method. Under hydrating conditions, the activated GRGDY could be directly immobilized to the surface exposed amine groups of the PLGA-NH2 blend films or scaffolds. For the PLGA blend films, the surface density of GRGDY, surface wettability change, and cell adhesion behaviors were characterized. The extent of cell adhesion was substantially enhanced by increasing the blend ratio of PLGA-NH2 to PLGA. The level of an alkaline phosphatase activity, measured as a degree of cell differentiation, was also enhanced as a result of the introduction of cell adhesive peptides.

Original languageEnglish
Pages (from-to)5613-5620
Number of pages8
JournalBiomaterials
Volume25
Issue number25
DOIs
StatePublished - Nov 2004
Externally publishedYes

Funding

This study was supported by the Korea Science and Engineering Foundation (R01-2003-000-10362-0) and the Korea Research Foundation (X01524), Korea.

Keywords

  • Adhesion
  • Polyactic acid
  • RGD peptide
  • Scaffold
  • Surface modification

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