Abstract
Bacillus subtilis lipase (BSL2) has been successfully immobilized into a Cu-BTC based hierarchically porous metal-organic framework material for the first time. The Cu-BTC hierarchically porous MOF material with large mesopore apertures is prepared conveniently by using a template-free strategy under mild conditions. The immobilized BSL2 presents high enzymatic activity and perfect reusability during the esterification reaction. After 10 cycles, the immobilized BSL2 still exhibits 90.7% of its initial enzymatic activity and 99.6% of its initial conversion.
| Original language | English |
|---|---|
| Pages (from-to) | 6998-7003 |
| Number of pages | 6 |
| Journal | Dalton Transactions |
| Volume | 45 |
| Issue number | 16 |
| DOIs | |
| State | Published - Apr 28 2016 |
| Externally published | Yes |
Funding
This work was supported by the National Natural Science Foundation of China (Grant No. 21171064, 21371067 and 21373095) and the Open Project of Key Laboratory of Polyoxometalate Science of Ministry of Education (130026513).