Immobilization of Arthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion

Tae Gwan Park; Allan S. Hoffman

doi:10.1007/BF01026779

Immobilization of Arthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion

Tae Gwan Park, Allan S. Hoffman

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19 Scopus citations

Abstract

Organic solvents have sometimes been used to increase the solubility of water insoluble substrates for steroid transformation using immobilized whole cells, even though the cell viability is often damaged. Polymeric surfactants which form micelles in aqueous solutions could be used instead of organic solvents to solubilize the steroid. We have successfully utilized this approach by employing a poly(dimethyl siloxane)-poly(ethyleneoxide) (PDMS-PEO) block copolymer surfactant to enhance conversion of hydrocortisone to prednisolone by immobilized Arthrobacter simplex cells, without deactivation of the immobilized cells.

Original language	English
Pages (from-to)	17-22
Number of pages	6
Journal	Biotechnology Letters
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/BF01026779
State	Published - Jan 1989
Externally published	Yes

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title = "Immobilization of Arthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion",

abstract = "Organic solvents have sometimes been used to increase the solubility of water insoluble substrates for steroid transformation using immobilized whole cells, even though the cell viability is often damaged. Polymeric surfactants which form micelles in aqueous solutions could be used instead of organic solvents to solubilize the steroid. We have successfully utilized this approach by employing a poly(dimethyl siloxane)-poly(ethyleneoxide) (PDMS-PEO) block copolymer surfactant to enhance conversion of hydrocortisone to prednisolone by immobilized Arthrobacter simplex cells, without deactivation of the immobilized cells.",

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AU - Hoffman, Allan S.

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Y1 - 1989/1

N2 - Organic solvents have sometimes been used to increase the solubility of water insoluble substrates for steroid transformation using immobilized whole cells, even though the cell viability is often damaged. Polymeric surfactants which form micelles in aqueous solutions could be used instead of organic solvents to solubilize the steroid. We have successfully utilized this approach by employing a poly(dimethyl siloxane)-poly(ethyleneoxide) (PDMS-PEO) block copolymer surfactant to enhance conversion of hydrocortisone to prednisolone by immobilized Arthrobacter simplex cells, without deactivation of the immobilized cells.

AB - Organic solvents have sometimes been used to increase the solubility of water insoluble substrates for steroid transformation using immobilized whole cells, even though the cell viability is often damaged. Polymeric surfactants which form micelles in aqueous solutions could be used instead of organic solvents to solubilize the steroid. We have successfully utilized this approach by employing a poly(dimethyl siloxane)-poly(ethyleneoxide) (PDMS-PEO) block copolymer surfactant to enhance conversion of hydrocortisone to prednisolone by immobilized Arthrobacter simplex cells, without deactivation of the immobilized cells.

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Immobilization of Arthrobacter simplex cells in thermally reversible hydrogels: Comparative effects of organic solvent and polymeric surfactant on steroid conversion

Abstract

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