Abstract
Aim: The aim of this work was to demonstrate and evaluate the analytical performance of coupling the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. Methodology & results: A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. Conclusion: This immediate drop on demand technology/open port sampling interface/ESI-MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 1667-1679 |
| Number of pages | 13 |
| Journal | Bioanalysis |
| Volume | 9 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 2017 |
Funding
The work of GJ Van Berkel and V Kertesz on the development of the concept for noncontact sample capture with the OPSI, the assembly of prototype interfaces for that purpose and droplet capture optimization studies were supported by the United States Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences and Biosciences Division. Support of GJ Van Berkel and V Kertesz for the particular small molecule and protein applications presented, and the loan of the QTRAP 5500 and the TripleTOF 5600+ mass spectrometers, were provided through a Cooperative Research and Development Agreement (CRADA NFE-10–02966) with Sciex. This manuscript has been authored by UT-Battelle, LLC under contract no. DE-AC05–00OR22725 with the U.S. Department of Energy. The United States Government retains copyright and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a nonexclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
| Funders | Funder number |
|---|---|
| United States Department of Energy | |
| Office of Science | |
| Basic Energy Sciences | |
| Chemical Sciences, Geosciences, and Biosciences Division |
Keywords
- drop dispensing
- electrospray ionization
- immediate drop on demand
- mass spectrometry
- microtiter plates
- noncontact dispensing
- open port sampling interface