Abstract
With the steady improvement of x-ray optics with high resolution and efficiency, and continued development or adaptation of different imaging and measuring techniques, soft x-ray microscopy has emerged as a powerful method to image and analyze fully hydrated specimens of several micrometer thickness at sub-optical resolution (for a recent overview, see ref. 1). We report on experiments performed with the cryo scanning transmission x-ray microscope (cryo-STXM), which has recently come into operation at the National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory. Cryo-STXM uses x-rays with energies between the absorption edge of Carbon (E = 284 eV) and Oxygen (E = 543 eV) from the soft x-ray undulator at the NSLS. Fully hydrated specimens such as eucaryotic cells in water or ice layers of up to 10 micrometer thickness can be imaged without any additional need for contrast enhancing techniques.
| Original language | English |
|---|---|
| Pages (from-to) | 354-355 |
| Number of pages | 2 |
| Journal | Microscopy and Microanalysis |
| Volume | 4 |
| DOIs | |
| State | Published - Jul 1 1998 |
| Externally published | Yes |
Funding
The authors gratefully acknowledge support from the Office of Biological and Environmental Research, U.S. DoE under contract DE-FG02-89ER60858, the National Science Foundation under grants DBI- 9605045 and ECS-9510499, and the Alexander von Humboldt-Foundation (Feodor-Lynen Fellowship to JM). This work was performed in part at the National Synchrotron Light Source which is operated by the Department on Energy.
| Funders | Funder number |
|---|---|
| National Science Foundation | DBI- 9605045, ECS-9510499 |
| U.S. Department of Energy | DE-FG02-89ER60858 |
| Alexander von Humboldt-Stiftung | |
| Biological and Environmental Research |