Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering

C. Harry Allen; Robyn Skillings; Duale Ahmed; Sarita C. Sanchez; Kaitlyn Altwasser; Justin Gagnon; Vinita Chauha; Edana Cassol; Sangeeta Murugkar

doi:10.1117/12.2650138

Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering

C. Harry Allen, Robyn Skillings, Duale Ahmed, Sarita C. Sanchez, Kaitlyn Altwasser, Justin Gagnon, Vinita Chauha, Edana Cassol, Sangeeta Murugkar

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 – 3100 cm^-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp X-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.

Original language	English
Title of host publication	Advanced Chemical Microscopy for Life Science and Translational Medicine 2023
Editors	Ji-Xin Cheng, Wei Min, Garth J. Simpson
Publisher	SPIE
ISBN (Electronic)	9781510658899
DOIs	https://doi.org/10.1117/12.2650138
State	Published - 2023
Externally published	Yes
Event	Advanced Chemical Microscopy for Life Science and Translational Medicine 2023 - San Francisco, United States Duration: Jan 28 2023 → Jan 30 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12392
ISSN (Print)	1605-7422

Conference

Conference	Advanced Chemical Microscopy for Life Science and Translational Medicine 2023
Country/Territory	United States
City	San Francisco
Period	01/28/23 → 01/30/23

Funding

We would like to thank Profs. Anatoli Ianoul and Jeff Manthorpe in the Department of Chemistry at Carleton University for providing the DMSO solution.

Keywords

chemical imaging
radiobiology
SRS microscopy

Access to Document

10.1117/12.2650138

Cite this

Allen, C. H., Skillings, R., Ahmed, D., Sanchez, S. C., Altwasser, K., Gagnon, J., Chauha, V., Cassol, E., & Murugkar, S. (2023). Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering. In J.-X. Cheng, W. Min, & G. J. Simpson (Eds.), Advanced Chemical Microscopy for Life Science and Translational Medicine 2023 Article 1239204 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12392). SPIE. https://doi.org/10.1117/12.2650138

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title = "Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering",

abstract = "Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 – 3100 cm-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp X-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.",

keywords = "chemical imaging, radiobiology, SRS microscopy",

author = "Allen, {C. Harry} and Robyn Skillings and Duale Ahmed and Sanchez, {Sarita C.} and Kaitlyn Altwasser and Justin Gagnon and Vinita Chauha and Edana Cassol and Sangeeta Murugkar",

note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; Advanced Chemical Microscopy for Life Science and Translational Medicine 2023 ; Conference date: 28-01-2023 Through 30-01-2023",

year = "2023",

doi = "10.1117/12.2650138",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Allen, CH, Skillings, R, Ahmed, D, Sanchez, SC, Altwasser, K, Gagnon, J, Chauha, V, Cassol, E & Murugkar, S 2023, Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering. in J-X Cheng, W Min & GJ Simpson (eds), Advanced Chemical Microscopy for Life Science and Translational Medicine 2023., 1239204, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12392, SPIE, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, San Francisco, United States, 01/28/23. https://doi.org/10.1117/12.2650138

Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering. / Allen, C. Harry; Skillings, Robyn; Ahmed, Duale et al.
Advanced Chemical Microscopy for Life Science and Translational Medicine 2023. ed. / Ji-Xin Cheng; Wei Min; Garth J. Simpson. SPIE, 2023. 1239204 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12392).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Allen, C. Harry

AU - Skillings, Robyn

AU - Ahmed, Duale

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AU - Gagnon, Justin

AU - Chauha, Vinita

AU - Cassol, Edana

AU - Murugkar, Sangeeta

PY - 2023

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N2 - Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 – 3100 cm-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp X-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.

AB - Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 – 3100 cm-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp X-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.

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Y2 - 28 January 2023 through 30 January 2023

ER -

Allen CH, Skillings R, Ahmed D, Sanchez SC, Altwasser K, Gagnon J et al. Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering. In Cheng JX, Min W, Simpson GJ, editors, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023. SPIE. 2023. 1239204. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2650138

Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering

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