Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

A. A. Puretzky; D. B. Geohegan; G. B. Hurst; M. V. Buchanan; B. S. Luky’anchuk

doi:10.1103/PhysRevLett.83.444

Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

A. A. Puretzky
, D. B. Geohegan
, G. B. Hurst
, M. V. Buchanan
, B. S. Luky’anchuk

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

Original language	English
Pages (from-to)	444-447
Number of pages	4
Journal	Physical Review Letters
Volume	83
Issue number	2
DOIs	https://doi.org/10.1103/PhysRevLett.83.444
State	Published - Jan 1 1999

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10.1103/PhysRevLett.83.444

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abstract = "The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.",

author = "Puretzky, \{A. A.\} and Geohegan, \{D. B.\} and Hurst, \{G. B.\} and Buchanan, \{M. V.\} and Luky{\textquoteright}anchuk, \{B. S.\}",

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AU - Puretzky, A. A.

AU - Geohegan, D. B.

AU - Hurst, G. B.

AU - Buchanan, M. V.

AU - Luky’anchuk, B. S.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

AB - The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

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U2 - 10.1103/PhysRevLett.83.444

DO - 10.1103/PhysRevLett.83.444

M3 - Article

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SP - 444

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JO - Physical Review Letters

JF - Physical Review Letters

IS - 2

ER -

Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

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