Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

A. A. Puretzky; D. B. Geohegan; G. B. Hurst; M. V. Buchanan; B. S. Luky’anchuk

doi:10.1103/PhysRevLett.83.444

Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

A. A. Puretzky, D. B. Geohegan, G. B. Hurst, M. V. Buchanan, B. S. Luky’anchuk

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Abstract

The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

Original language	English
Pages (from-to)	444-447
Number of pages	4
Journal	Physical Review Letters
Volume	83
Issue number	2
DOIs	https://doi.org/10.1103/PhysRevLett.83.444
State	Published - Jan 1 1999

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10.1103/PhysRevLett.83.444

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abstract = "The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.",

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AU - Hurst, G. B.

AU - Buchanan, M. V.

AU - Luky’anchuk, B. S.

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AB - The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

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Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

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