Abstract
The most sensitive analytical techniques available today for detecting immuno assay complexes are radio or enzyme immuno analytical techniques, by which quantities of 107-108 analyte molecules can be detected. With the introduction of scanning force microscopy, a new method for detecting biological processes became available. Here, we examine the feasibility of using scanning force microscopy as a biosensitive tool. We demonstrate that single or multiple rabbit anti-human serum albumin molecules form complexes with preadsorbed single human serum albumin molecules on mica. However, no interaction is observed between human immunoglobulin G molecules and preadsorbed single albumin molecules; only separate antigens and antibodies are observed at random positions on the mica. This shows the ability of scanning force microscopy to act as a biosensor for detection of immunocomplexes, and to act as a very powerful tool to study molecule- surface interactions in general.
Original language | English |
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Pages (from-to) | 395-400 |
Number of pages | 6 |
Journal | Scanning Microscopy |
Volume | 9 |
Issue number | 2 |
State | Published - 1995 |
Externally published | Yes |
Keywords
- Scanning force microscopy
- antibody-antigen interaction
- immune- complexes
- molecular adsorption