Identification of antioxidant phenolic compounds in feverfew (Tanacetum parthenium) by HPLC-ESI-MS/MS and NMR

Changqing Wu, Feng Chen, Xi Wang, Yonnie Wu, Meidul Dong, Guoqing He, Ronald D. Galyean, Lilin He, Guohui Huang

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Antioxidant polyphenolic acids in the medicinal herb feverfew (Tanacetum parthenium) were isolated through in vitro bioassay-orientated antioxidant tests in response to 1,1-diphenyl-2-picrylhydrazyl (DPPH•,) free radical scavenging and Fe2+-chelating activities. Purification of the active compounds and their structural elucidation involved a variety of techniques including open-column chromatography, HPLC, GC-MS, LC-MS and NMR. Major compounds with potent DPPH•, scavenging activities were characterlsed as 3,5-, 4,5- and 3,4-di-O-caffeoylquinic acids (DCQAs). This is the first report of DCQAs found in feverfew.

Original languageEnglish
Pages (from-to)401-410
Number of pages10
JournalPhytochemical Analysis
Volume18
Issue number5
DOIs
StatePublished - Sep 2007
Externally publishedYes

Keywords

  • Antioxidant
  • Dicaffeoylquinic acid
  • Feverfew
  • LC-MS
  • NMR
  • Phenolics

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