Abstract
In many dioecious plants, gender influences economic value, breeding schemes, and/or opportunities for commercial use of genetically transformed materials. The objective of this study was to identify molecular markers linked to sex determination loci in the dioecious plant Salix viminalis L. A 4 x 4 factorial mating design was used to identify sex ratios in full- sibling progeny, to generate a working genetics model for segregating sex ratios, and to search for molecular markers linked to sex determination genes. Bulked segregant analysis, utilizing 380 arbitrary decamer primers to generate randomly amplified polymorphic DNA (RAPD) products was initially applied to progeny sets from 3 of the 16 full-sibling families. Of the 1080 RAPD bands examined, only a single 560 bp band was shown to he linked to a sex determination locus. The same 560 bp band occurred in three additional fullsibling families and was present in one female parent and one male parent involved in the factorial mating design. This marker, UBC354560, is biparentally inherited, is associated with femaleness in certain genetic backgrounds, and is linked to allele A1 in the proposed two-locus epistatic genetic model of sex determination for S. viminalis. Southern blots confirmed marker homology among progeny and parents used in this study.
| Original language | English |
|---|---|
| Pages (from-to) | 44-49 |
| Number of pages | 6 |
| Journal | Journal of Heredity |
| Volume | 89 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1998 |
Funding
From the Departments of Plant Breeding (Alstrom-Ra-paport) and Forest Genetics (Lascoux), Swedish University of Agricultural Sciences, Uppsala, Sweden, and the Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422 (Wang, Roberts, and Tuskan). The authors wish to thank Urban Pettersson and Helena Overholt for help with field work, and Urban Gullberg for initiating this project and for his continued support. We are grateful for the suggestions made on earlier versions of this manuscript by Leslie Saidak, Stan Wullschleger, Lee Gunter, Hans van Buijtenen, Barbara McCutchen, Jos van Damme, and Ulf Lagercrantz. The authors acknowledge the Swedish National Energy Commission (NUTEK) for financial support for the field research and data analysis portions of this study and the U.S. Department of Energy, Biofuels Systems Division, under contract with Lockheed Martin Energy Research Corp., contract no. DE-AC05–96OR22464, for supporting the laboratory research portion of this study. Address reprint requests to G. A. Tuskan at the address above. EDS Publication #4716.