Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering

Hyuk Sang Yoo, Eun Ah Lee, Jun Jin Yoon, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

Hyaluronic acid (hyaluronan, HA) was immobilized onto the surface of macroporous biodegradable poly(D,L-lactic acid-co-glycolic acid) [PLGA] scaffolds to enhance the attachment, proliferation, and differentiation of chondrocytes for cartilage tissue engineering. The PLGA scaffolds were prepared by blending PLGA with varying amounts of amine-terminated PLGA-PEG di-block copolymer. They were fabricated by a gas foaming/salt leaching method. HA was chemically conjugated to the surface-exposed amine groups on the pre-fabricated scaffolds. The amount of surface exposed free amine groups was quantitatively determined by conjugating an amine-reactive fluorescent dye to the PLGA blend films. The extent of HA immobilization was also confirmed by measuring water contact angles. When chondrocytes were seeded within HA modified PLGA scaffolds, enhanced cellular attachment was observed compared to unmodified PLGA scaffolds. Furthermore, glycosaminoglycan and total collagen synthesis increased substantially for HA modified PLGA scaffolds. RT-PCR result and histological examination of the resultant cartilage tissue revealed that HA modified scaffolds excelled in inducing cartilage tissue formation in terms of collagen type II expression and tissue morphological characteristics.

Original languageEnglish
Pages (from-to)1925-1933
Number of pages9
JournalBiomaterials
Volume26
Issue number14
DOIs
StatePublished - May 2005
Externally publishedYes

Funding

This study was supported the grants from the Korea Research Foundation (R11-1997-044-06002-0) and from the Korea Science and Engineering Foundation (R01-2003-000-10362-0), Korea.

Keywords

  • Chondrocyte
  • Hyaluronic acid
  • Polylactic acid
  • Scaffold
  • Surface modification

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